Wow 🤩! Amazing! Many congratulations Omaya and Team!

4/ Huge thanks to co-authors Sudipta, @jayantik.bsky.social, Jeevan & Amruta.
Grateful to @sshekhr.bsky.social lab for support throughout, and to the @PLOSGenetics editors & reviewers for their thoughtful feedback 🙏

3/ Strikingly, the “minor” isoform Tpm2 can fully substitute for Tpm1 to protect & organize actin cables.
This supports a concentration-dependent, formin-independent model of Tpm binding and reveals unexpected redundancy between paralogs.

2/ We show that Tpm1 & Tpm2 protect equally well from cofilin-mediated actin filament severing in collab
@sshekhr.bsky.social lab and Sudipta.
Despite Tpm1 being ~5–6x more abundant, both share overlapping biochemical activities, revealing Tpm2’s latent protective role in cells.

1/ New functional tropomyosin probes, we show that Tpm1&Tpm2 colocalize on actin cables and bind filaments nucleated by either formin challenging the long-held model of strict Tpm–formin pairing. Tpm levels also regulate endocytosis by balancing linear & branched actin networks.

Thank you very much, Nika.

#collaboration with the fantastic labs of Gayathri, Vinoth, @kuruhoornambi, @iamsamayp. Funded and supported by @India_Alliance, @DBTIndia, @ANRFIndia, @serbonline, @iiscbangalore, @IISERPune, @NCBS_Bangalore & @niserbbs

Thanks, Sophie!

Thanks

Thanks very much Felipe! ALFA made it all click-always fun to work with!

Thanks, Mara! The ALFA tag/NbALFA combo is a game-changer!

Thank you very much Omaya!