3/ Strikingly, the “minor” isoform Tpm2 can fully substitute for Tpm1 to protect & organize actin cables.
This supports a concentration-dependent, formin-independent model of Tpm binding and reveals unexpected redundancy between paralogs.

2/ We show that Tpm1 & Tpm2 protect equally well from cofilin-mediated actin filament severing in collab
@sshekhr.bsky.social lab and Sudipta.
Despite Tpm1 being ~5–6x more abundant, both share overlapping biochemical activities, revealing Tpm2’s latent protective role in cells.

1/ New functional tropomyosin probes, we show that Tpm1&Tpm2 colocalize on actin cables and bind filaments nucleated by either formin challenging the long-held model of strict Tpm–formin pairing. Tpm levels also regulate endocytosis by balancing linear & branched actin networks.

How do cells keep their #actin “highways” stable?🚦
Our paper @PLOS Genetics reveals surprising teamwork (and backup plans!) between tropomyosin isoforms in yeast. #imaging #cytoskeleton #celldivision
This work was brilliantly led by @anubhavdhar.bsky.social @bagyasree.bsky.social
t.co/RvUrzad5tL

🎉🍾Excited to share our #Asgardarchaea #odin #cytoskeleton study published in
@embojournal.org Dual FtsZ proteins in Odinarchaeon form distinct filaments shedding light on tubulin/FtsZ evolution. Brilliantly led by @jayantik.bsky.social
www.embopress.org/doi/full/10....

This method reveals actin patches, cables, rings (yeast), and cortex, filopodia, lamellipodia, stress fibers (cell lines). Notably, it captures nuclear actin filaments with high specificity highlighting IntAct-U-ExM’s power to resolve nanoscale actin architecture across species.

Preprint: IntAct-U-ExM enables robust, isoform-specific expansion microscopy of actin networks in yeast and mammalian cells. A cool study led by @anubhavdhar.bsky.social in collaboration with Sudarshan and Deepak Nair. www.biorxiv.org/content/10.1...

First Post! Thrilled to share our lab's first evolutionary cytoskeleton study! We uncover two distinct FtsZ paralogs in Odinarchaeota OdinFtsZ1 forms curved filaments, while OdinFtsZ2 assembles into spiral rings. A glimpse into tubulin evolution! threads👇 #Eukaryogenesis #AsgardArchaea #cytoskeleton