Filippo Caschera
@stjle.bsky.social
Synthetic Biology, Artificial Cells, Amphiphile Systems, Machine Learning, Cell-Free Protein Synthesis: Italy, Denmark, Japan, USA, France, Germany, UK.
Cell-Free Protein Synthesis consultant
https://scholar.google.com/citations?user=u6OhA2oAAAAJ&hl=en
Cell-Free Protein Synthesis consultant
https://scholar.google.com/citations?user=u6OhA2oAAAAJ&hl=en
Pinned
Filippo Caschera
@stjle.bsky.social
· Feb 19
Cell-free protein synthesis platforms for accelerating drug discovery
Cell-free protein synthesis is a platform for streamlined production of macromolecules. Recently, several proteins with pharmaceutical relevance were …
www.sciencedirect.com
Pleased to share my latest review on Cell-Free Protein Synthesis #CFPS and #AI/ML for accelerating drug discovery
www.sciencedirect.com/science/arti...
#CellFree #SynBio
www.sciencedirect.com/science/arti...
#CellFree #SynBio
Genome synthesis, assembly, and rebooting of therapeutically useful high G+C% mycobacteriophages | PNAS www.pnas.org/doi/10.1073/...
Genome synthesis, assembly, and rebooting of therapeutically useful high G+C% mycobacteriophages | PNAS
Bacteriophages show therapeutic promise for treating bacterial pathogens including
nontuberculous mycobacteria (NTM). A major impediment is the pau...
www.pnas.org
November 12, 2025 at 4:24 PM
Genome synthesis, assembly, and rebooting of therapeutically useful high G+C% mycobacteriophages | PNAS www.pnas.org/doi/10.1073/...
Synthetic Rewiring of Virus-like Particles via Circular Permutation Enables Modular Peptide Display and Protein Encapsulation | ACS Nano pubs.acs.org/doi/10.1021/...
Synthetic Rewiring of Virus-like Particles via Circular Permutation Enables Modular Peptide Display and Protein Encapsulation
Virus-like particles (VLPs) are self-assembling nanoparticles derived from viruses with the potential as scaffolds for myriad applications. They are also excellent testbeds for engineering protein sup...
pubs.acs.org
November 1, 2025 at 4:47 PM
Synthetic Rewiring of Virus-like Particles via Circular Permutation Enables Modular Peptide Display and Protein Encapsulation | ACS Nano pubs.acs.org/doi/10.1021/...
Reposted by Filippo Caschera
Reposted by Filippo Caschera
QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in GUVs https://www.biorxiv.org/content/10.1101/2025.10.23.684161v1
October 24, 2025 at 3:06 AM
QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in GUVs https://www.biorxiv.org/content/10.1101/2025.10.23.684161v1
Automated Cell-free Protein Synthesis for Distributed Biomanufacturing
www.medrxiv.org/content/10.1...
www.medrxiv.org/content/10.1...
Automated Cell-free Protein Synthesis for Distributed Biomanufacturing
Recombinant proteins are fundamental to modern medicine, enabling research, diagnostics, and drug discovery, yet their access is often restricted by centralized manufacturing and cold-chain distributi...
www.medrxiv.org
October 18, 2025 at 5:30 AM
Automated Cell-free Protein Synthesis for Distributed Biomanufacturing
www.medrxiv.org/content/10.1...
www.medrxiv.org/content/10.1...
Reposted by Filippo Caschera
Assembly and cell-free expression of a partial genome for the synthetic cell https://www.biorxiv.org/content/10.1101/2025.10.16.682769v1
October 16, 2025 at 9:04 PM
Assembly and cell-free expression of a partial genome for the synthetic cell https://www.biorxiv.org/content/10.1101/2025.10.16.682769v1
Metabolomics-informed coarse-grained model enables prediction of cell-free protein expression dynamics www.biorxiv.org/content/10.1...
Metabolomics-informed coarse-grained model enables prediction of cell-free protein expression dynamics
Cell-free expression systems have gained considerable attention for their potential in biomanufac-turing, biosensing, and circuit prototyping. All these endeavors make use of the innate metabolic acti...
www.biorxiv.org
October 13, 2025 at 4:46 AM
Metabolomics-informed coarse-grained model enables prediction of cell-free protein expression dynamics www.biorxiv.org/content/10.1...
Generative design of antibody Fc-variants with synthetic and programmable functional profiles
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Generative design of antibody Fc-variants with synthetic and programmable functional profiles
Beyond antigen recognition, antibodies direct diverse immune effector functions through their constant (Fc) domain. While the Fc domain is central to antibody biology and therapeutic efficacy, our und...
www.biorxiv.org
October 12, 2025 at 11:07 AM
Generative design of antibody Fc-variants with synthetic and programmable functional profiles
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
A generative artificial intelligence approach for the discovery of antimicrobial peptides against multidrug-resistant bacteria www.nature.com/articles/s41...
A generative artificial intelligence approach for the discovery of antimicrobial peptides against multidrug-resistant bacteria - Nature Microbiology
This study presents a generative artificial intelligence approach for the high-throughput discovery of antimicrobials against multidrug-resistant bacteria.
www.nature.com
October 3, 2025 at 11:10 AM
A generative artificial intelligence approach for the discovery of antimicrobial peptides against multidrug-resistant bacteria www.nature.com/articles/s41...
Reposted by Filippo Caschera
Split inteins for generating combinatorial non-ribosomal peptide libraries https://www.biorxiv.org/content/10.1101/2025.10.02.680031v1
October 3, 2025 at 4:17 AM
Split inteins for generating combinatorial non-ribosomal peptide libraries https://www.biorxiv.org/content/10.1101/2025.10.02.680031v1
Reposted by Filippo Caschera
Cell-free opens a unique test-bed to reconstituteand to explore alternative life possibilities.
Check our review (by Maud Hofmann, Farouk Abdo, @manishmicrobe and myself) :
Reconstituting alternative life using the test-bed of cell-free systems
doi.org/10.1098/rstb...
Check our review (by Maud Hofmann, Farouk Abdo, @manishmicrobe and myself) :
Reconstituting alternative life using the test-bed of cell-free systems
doi.org/10.1098/rstb...
October 2, 2025 at 10:58 AM
Cell-free opens a unique test-bed to reconstituteand to explore alternative life possibilities.
Check our review (by Maud Hofmann, Farouk Abdo, @manishmicrobe and myself) :
Reconstituting alternative life using the test-bed of cell-free systems
doi.org/10.1098/rstb...
Check our review (by Maud Hofmann, Farouk Abdo, @manishmicrobe and myself) :
Reconstituting alternative life using the test-bed of cell-free systems
doi.org/10.1098/rstb...
Rational design of a hospital-specific phage cocktail to treat Enterobacter cloacae complex infections www.nature.com/articles/s41...
Rational design of a hospital-specific phage cocktail to treat Enterobacter cloacae complex infections - Nature Microbiology
The Entelli-02 phage product containing five phages has frontline potential to address infections caused by the multidrug-resistant Enterobacter cloacae complex.
www.nature.com
September 30, 2025 at 3:51 AM
Rational design of a hospital-specific phage cocktail to treat Enterobacter cloacae complex infections www.nature.com/articles/s41...
Improved Therapeutic Antibody Reformatting through Multimodal Machine Learning
arxiv.org/abs/2509.196...
arxiv.org/abs/2509.196...
Improved Therapeutic Antibody Reformatting through Multimodal Machine Learning
Modern therapeutic antibody design often involves composing multi-part assemblages of individual functional domains, each of which may be derived from a different source or engineered independently. W...
arxiv.org
September 28, 2025 at 8:51 PM
Improved Therapeutic Antibody Reformatting through Multimodal Machine Learning
arxiv.org/abs/2509.196...
arxiv.org/abs/2509.196...
Cell-free recombinase-integrated Boolean output system: Cell Systems www.cell.com/cell-systems...
Cell-free recombinase-integrated Boolean output system
Chen et al. present CRIBOS, a large-scale recombinase-based logic platform for cell-free
systems. They define key circuit design rules, demonstrate portable paper-based logic
devices, and enable long-...
www.cell.com
September 24, 2025 at 3:56 PM
Cell-free recombinase-integrated Boolean output system: Cell Systems www.cell.com/cell-systems...
Reposted by Filippo Caschera
Reposted by Filippo Caschera
Hollow-fibre biomanufacturing and cell-free engineering of HEK293 extracellular vesicles https://www.biorxiv.org/content/10.1101/2025.09.20.677537v1
September 21, 2025 at 11:04 PM
Hollow-fibre biomanufacturing and cell-free engineering of HEK293 extracellular vesicles https://www.biorxiv.org/content/10.1101/2025.09.20.677537v1
Generative design of novel bacteriophages with genome language models www.biorxiv.org/content/10.1...
Generative design of novel bacteriophages with genome language models
Many important biological functions arise not from single genes, but from complex interactions encoded by entire genomes. Genome language models have emerged as a promising strategy for designing biol...
www.biorxiv.org
September 17, 2025 at 6:47 PM
Generative design of novel bacteriophages with genome language models www.biorxiv.org/content/10.1...
Microsystem technologies for accelerating the discovery and translation of immunotherapies www.nature.com/articles/s41...
Microsystem technologies for accelerating the discovery and translation of immunotherapies - Nature Reviews Drug Discovery
The potential of microsystem technologies to accelerate the development and clinical translation of immunotherapies is now being realized. This Review discusses recent advances in microsystem technolo...
www.nature.com
September 15, 2025 at 4:36 PM
Microsystem technologies for accelerating the discovery and translation of immunotherapies www.nature.com/articles/s41...
Reposted by Filippo Caschera
Reposted by Filippo Caschera
Characterizing Cell-Free Transcription and Translation Dynamics with Nucleic Acid-Based Assays https://www.biorxiv.org/content/10.1101/2025.09.10.675373v1
September 11, 2025 at 5:04 AM
Characterizing Cell-Free Transcription and Translation Dynamics with Nucleic Acid-Based Assays https://www.biorxiv.org/content/10.1101/2025.09.10.675373v1
Development of a nonreplicative phage-based DNA delivery system and its application to antimicrobial therapies:
academic.oup.com/pnasnexus/ar...
academic.oup.com/pnasnexus/ar...
Validate User
academic.oup.com
September 5, 2025 at 3:10 PM
Development of a nonreplicative phage-based DNA delivery system and its application to antimicrobial therapies:
academic.oup.com/pnasnexus/ar...
academic.oup.com/pnasnexus/ar...
Reposted by Filippo Caschera
How safe is your mind in a world flooded with junk information? Yuval Noah Harari warns that unlike past media, AI-driven networks never rest, blurring the boundaries between us and digital reality. He calls to preserve your autonomy as AI becomes more unpredictable www.youtube.com/watch?v=K1Ov...
September 2, 2025 at 11:03 AM
How safe is your mind in a world flooded with junk information? Yuval Noah Harari warns that unlike past media, AI-driven networks never rest, blurring the boundaries between us and digital reality. He calls to preserve your autonomy as AI becomes more unpredictable www.youtube.com/watch?v=K1Ov...