Srijayee Ghosh
@srijayee-ghosh.bsky.social
Figuring out fluorescence 🧐|
Super-resolution microscopy🌟 |smFRET🟢🔴| DNA-PAINT 🧬| Nanotechnology 🧪|
Postdoc in Biomolecular Nanodynamics lab at UniBasel 👩🏻🔬
Super-resolution microscopy🌟 |smFRET🟢🔴| DNA-PAINT 🧬| Nanotechnology 🧪|
Postdoc in Biomolecular Nanodynamics lab at UniBasel 👩🏻🔬
Can one use 'Dark' or non-emissive dyes for #fast #Super-Resolution #fluorescence 🌟 #microscopy?
In this work, we show the first proof-of-concept of Dark-RET (DRET) #DNA-PAINT, using a dark donor with a bright acceptor, for cellular imaging in <30 seconds. Link ⬇️
pubs.acs.org/doi/abs/10.1...
In this work, we show the first proof-of-concept of Dark-RET (DRET) #DNA-PAINT, using a dark donor with a bright acceptor, for cellular imaging in <30 seconds. Link ⬇️
pubs.acs.org/doi/abs/10.1...
Using Dark Dyes for Fast Super-Resolution Imaging: A Proof-of-Concept Study
DNA-PAINT (Point Accumulation for Imaging in Nanoscale Topography) exploits the transient hybridization of a short fluorescent DNA imager strand to a complementary docking strand to achieve super-reso...
pubs.acs.org
November 2, 2025 at 10:40 PM
Can one use 'Dark' or non-emissive dyes for #fast #Super-Resolution #fluorescence 🌟 #microscopy?
In this work, we show the first proof-of-concept of Dark-RET (DRET) #DNA-PAINT, using a dark donor with a bright acceptor, for cellular imaging in <30 seconds. Link ⬇️
pubs.acs.org/doi/abs/10.1...
In this work, we show the first proof-of-concept of Dark-RET (DRET) #DNA-PAINT, using a dark donor with a bright acceptor, for cellular imaging in <30 seconds. Link ⬇️
pubs.acs.org/doi/abs/10.1...
Reposted by Srijayee Ghosh
Done ✅: the www.nanodynamicslab.nl website is no more: one year after our move from the Netherlands to Switzerland (the 'Highlands'? 🤔), it was time to take the old website down.
You still find us here, at our @unibas.ch home: schmid.chemie.unibas.ch/en/ (revamp underway).
You still find us here, at our @unibas.ch home: schmid.chemie.unibas.ch/en/ (revamp underway).
Home | Research Group Schmid | University of Basel
schmid.chemie.unibas.ch
January 31, 2025 at 5:14 PM
Done ✅: the www.nanodynamicslab.nl website is no more: one year after our move from the Netherlands to Switzerland (the 'Highlands'? 🤔), it was time to take the old website down.
You still find us here, at our @unibas.ch home: schmid.chemie.unibas.ch/en/ (revamp underway).
You still find us here, at our @unibas.ch home: schmid.chemie.unibas.ch/en/ (revamp underway).
Reposted by Srijayee Ghosh
I am super happy to share our latest article entitled "Decoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy", now published in Science www.science.org/doi/10.1126/...
It was my postdoctoral work mit @sauer-lab.bsky.social Congrats to all the co-authors!
It was my postdoctoral work mit @sauer-lab.bsky.social Congrats to all the co-authors!
January 10, 2025 at 2:07 PM
I am super happy to share our latest article entitled "Decoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy", now published in Science www.science.org/doi/10.1126/...
It was my postdoctoral work mit @sauer-lab.bsky.social Congrats to all the co-authors!
It was my postdoctoral work mit @sauer-lab.bsky.social Congrats to all the co-authors!
Reposted by Srijayee Ghosh
Check out a preprint from our lab! We've uncovered all possible speed imager sequences for DNA-PAINT. You can now do 10-plex imaging in just a few hours with simple Exchange-PAINT. 🚀📸 #DNApaint #SuperResolution
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
High-speed 10-plex DNA-PAINT with a larger sequence repertoire
DNA-Points Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) enables multiplexed super-resolution imaging of biological samples. We expand the repertoire of speed-optimized DNA sequences fo...
www.biorxiv.org
January 3, 2025 at 5:57 AM
Check out a preprint from our lab! We've uncovered all possible speed imager sequences for DNA-PAINT. You can now do 10-plex imaging in just a few hours with simple Exchange-PAINT. 🚀📸 #DNApaint #SuperResolution
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Srijayee Ghosh
What a way to start 2025! Our review on the Known Unknowns of the Hsp90 Chaperone has been published!
Huge thanks to Laura Silbermann and Benjamin Vermeer for all of their efforts, and to @scisonja.bsky.social for the great collaboration :)
You can read it here:
elifesciences.org/articles/102...
Huge thanks to Laura Silbermann and Benjamin Vermeer for all of their efforts, and to @scisonja.bsky.social for the great collaboration :)
You can read it here:
elifesciences.org/articles/102...
The known unknowns of the Hsp90 chaperone
An exploration of the unanswered questions in how the molecular chaperone Hsp90 supports protein homeostasis, and how single-molecule techniques could drive future breakthroughs in answering them.
elifesciences.org
January 1, 2025 at 9:35 AM
What a way to start 2025! Our review on the Known Unknowns of the Hsp90 Chaperone has been published!
Huge thanks to Laura Silbermann and Benjamin Vermeer for all of their efforts, and to @scisonja.bsky.social for the great collaboration :)
You can read it here:
elifesciences.org/articles/102...
Huge thanks to Laura Silbermann and Benjamin Vermeer for all of their efforts, and to @scisonja.bsky.social for the great collaboration :)
You can read it here:
elifesciences.org/articles/102...
A welcome move to boost Indian research.
The Indian government has announced a giant deal with multiple publishers that will allow an estimated 18 million students, faculty, and researchers free access to nearly 13,000 journals through a single portal. scim.ag/49vrG8c
India takes out giant nationwide subscription to 13,000 journals
Deal allows scholars to read paywalled articles for free and will cover open-access fees
scim.ag
December 12, 2024 at 3:55 AM
A welcome move to boost Indian research.
Reposted by Srijayee Ghosh
Fluorogenic binders would enable DyeCycling on regular coverslips – instead of the zero-mode waveguides that we use so far. We discuss the pros & cons of a range of fluorogenic systems in the context of #DyeCycling and #smFRET experiments.
Comments welcome!
Comments welcome!
December 10, 2024 at 5:45 PM
Fluorogenic binders would enable DyeCycling on regular coverslips – instead of the zero-mode waveguides that we use so far. We discuss the pros & cons of a range of fluorogenic systems in the context of #DyeCycling and #smFRET experiments.
Comments welcome!
Comments welcome!
Our Perspective article where @scisonja.bsky.social and I discuss the potential of #fluorogenic systems for #DyeCycling in #smFRET is now out in QRB Dicovery.
Link: doi.org/10.1017/qrd....
Link: doi.org/10.1017/qrd....
Srijayee & my perspective on
The potential of #fluorogenicity for single molecule #FRET and #DyeCycling
is now out in QRB Discovery as part of the Special Collection on Single Molecule Challenges in the 21st Century,
Guest edited by Felix Ritort, Giulia Palermo and Fredrik Westerlund.
The potential of #fluorogenicity for single molecule #FRET and #DyeCycling
is now out in QRB Discovery as part of the Special Collection on Single Molecule Challenges in the 21st Century,
Guest edited by Felix Ritort, Giulia Palermo and Fredrik Westerlund.
December 11, 2024 at 10:36 AM
Our Perspective article where @scisonja.bsky.social and I discuss the potential of #fluorogenic systems for #DyeCycling in #smFRET is now out in QRB Dicovery.
Link: doi.org/10.1017/qrd....
Link: doi.org/10.1017/qrd....