6/ We then asked how inherited TCR variation shapes core aspects of T cell function. By genotyping TCR chains at single-cell resolution, we found TCR polymorphism alters thymic lineage commitment, influences T cell responses in diseased tissues, and modulates surface expression of the TCR complex.

2/ Using recent public long-read sequencing data from over 2600 donors, we built a nearly-complete map of common genetic variation in TCR genes. We discovered hundreds of new V and J gene alleles, and found population-level amino acid variation at almost every single position within Vα/β genes.