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We have cloned several of this size for replicative (low copy) in gram negative but integrative in gram positives. The homolog arms and double antibiotic resistance cassettes makes them quite big though. Might be worth considering integrase mediated genome integrations for these purposes.

3/3 The destruction of peat swamp forest is of particular concern as they are not only habitat for a number of species but also function as carbon sinks. Their destruction can lead to the release of billions tons of CO2.

Let's do what we can to protect these wonderful species and their habitats ♥️

2/3 Orangutans are threatened by poachers, illegal wildlife trade and habitat loss. Humans increasing demand for fatty acids used not only in food, but also in various products and as first generation biodiesel is nowadays the greatest threat to the existence of our close cousin.

Wonder which would take longer: Mediaformulation or the actual reduction of the genome.

Beautiful 💚

Just took a look, and indeed, it looks awesome. Thank you 🙏