The timing of the rescue intervention was critical for embryoid body differentiation. Early rescue in mESCs led to better outcomes, while delaying it until after differentiation initiation intensely worsened molecular and cellular effects.

Signs of genomic instability were detected, along with morphological and functional responses.

Transposable elements became permanently de-repressed, with evidence of potential novel chimeric transcripts being generated.

Analyzing the impact on known imprinted regions and their unique properties enabled the discovery of 20 regions with imprinted-like epigenetic and regulatory signatures.

Transient loss of DNMT1 profoundly impacted multiple layers of epigenetic regulation, including DNA methylation, five key histone modifications, and gene expression.

Using mESCs, we showed that rescuing DNMT1 failed to fully reverse the repercussions of its loss on a broad molecular and functional scale. Only partial reversal could be achieved; many effects were permanent, and some arose from the rescue intervention itself.

Merry Christmas and happy holidays everyone!

Secret Santa with a twist…people can steal my gift if people get me to say one of my favourite words…

Thank you Belize! ☀️🌊🏝️🐚🐠 I had a blast. Now back to Montreal ❄️🥶

Random texts, emails and slack messages from lab folks of data makes my day, and gets me excited for our next lab meetings! Like this one just received. Cell phone snap shot of immunostaining of iPSC-derived cortical #organoid!