Beyond commentary on Tfh subsets and GC biology, expect plenty of bread and baking-related content from this Bluesky page going forward - my sourdough starter is even older than my current year of MD/PhD training!

Lastly, please reach out if you have any ideas or questions regarding our preprint!

And don't forget about the supplementary section! bit.ly/48HftyM

Beyond supporting data and figures, there we describe our bioinformatic methods in depth to aid open communication and reproducibility. Code and data will also soon be on GitHub and Zenodo for reanlaysis:
bit.ly/4q8nkuX
bit.ly/3L3v6r5

Further, we received key samples and insights from the Romberg and Henrickson Labs at CHOP. I also want to thank my thesis committee for their guidance: Sarah Henrickson, Taku Kambayashi, David Hill, and Chris Hunter.

@romberglab.bsky.social
@sarahhenrickson.bsky.social
@kingofpathogens.bsky.social

I have been lucky to work alongside my coauthors in both the Oldridge and Vella Labs, including Amy Baxter who is now leading a new group at I3H!

@derek-oldridge.bsky.social
@laura-a-vella.bsky.social
@amylizbaxter.bsky.social
@pennmedcso.bsky.social
@chopresearch.bsky.social
@i3h-upenn.bsky.social

Further, we use our high-plex data to ask how several clinically important axes of Tfh variation are integrated within single Tfh states, such as polarity (e.g. Tfh1 vs Tfh2 vs Tfh17) and Tem vs Tcm-like phenotypes. If you want to try every scoop in the parlor, these data are for you bit.ly/3MvcjFH

Through reanalysis of published vaccination scRNAseq, rheumatoid arthritis GWAS, and T cell eQTL data, we propose roles for elevated GNG4 expression in human immune health and disease. Here I would like to thank @saorisakaue.bsky.social and colleagues for discussing their study! bit.ly/4pX9bBj

Even more surprising was when Melanie Mumau and the Fajgenbaum Lab approached us with new data from lymph nodes of patients with Castleman disease - in this context, both Tfh positioning and GNG4 expression were highly dysregulated. Check out their preprint for more!

@davidfajgenbaum.bsky.social

Using spatially resolved and multimodal single-cell analyses of human tonsil and blood, we unexpectedly converged upon GNG4 as a distinguishing feature of GC-localized Tfh. GNG4 encodes G protein subunit gamma 4, a modulator of GPCR signaling most known for its roles in neurons and cancer biology.