Oleksandra Sasha Fanari
@sashafanari.bsky.social
PhD Candidate in BIOE at Northeastern University
RNA ~ RNA mods ~ pseudouridine
RNA ~ RNA mods ~ pseudouridine
Reposted by Oleksandra Sasha Fanari
1/6 🧬New paper at @cp-cellsystems.bsky.social led by @sashafanari.bsky.social! Does pseudouridine (Ψ) dynamically respond to cell state? We used nanopore DRS + Mod-p ID to map Ψ in neuron-like cells under “normal” and “perturbed” conditions (healthy differentiation and unhealthy Pb2+ poisoning).
March 21, 2025 at 3:42 PM
1/6 🧬New paper at @cp-cellsystems.bsky.social led by @sashafanari.bsky.social! Does pseudouridine (Ψ) dynamically respond to cell state? We used nanopore DRS + Mod-p ID to map Ψ in neuron-like cells under “normal” and “perturbed” conditions (healthy differentiation and unhealthy Pb2+ poisoning).
Reposted by Oleksandra Sasha Fanari
🧪🧬👩🔬🖥️
Do immortalized cells have a different RNA modification profile compared to primary cells? 🤔
@sashafanari.bsky.social @wanunupore.bsky.social @srouhanifard.bsky.social assess the most abundant RNA modification, pseudouridine, in primary and immortalized human T cells
👇
doi.org/10.1101/2025...
Do immortalized cells have a different RNA modification profile compared to primary cells? 🤔
@sashafanari.bsky.social @wanunupore.bsky.social @srouhanifard.bsky.social assess the most abundant RNA modification, pseudouridine, in primary and immortalized human T cells
👇
doi.org/10.1101/2025...
Direct RNA sequencing of primary human T cells reveals the impact of immortalization on mRNA pseudouridine modifications.
Immortalized cell lines are commonly used as proxies for primary cells in human biology research. For example, Jurkat leukemic T cells fundamentally contributed to uncovering T cell signaling, activat...
doi.org
March 10, 2025 at 8:49 PM
🧪🧬👩🔬🖥️
Do immortalized cells have a different RNA modification profile compared to primary cells? 🤔
@sashafanari.bsky.social @wanunupore.bsky.social @srouhanifard.bsky.social assess the most abundant RNA modification, pseudouridine, in primary and immortalized human T cells
👇
doi.org/10.1101/2025...
Do immortalized cells have a different RNA modification profile compared to primary cells? 🤔
@sashafanari.bsky.social @wanunupore.bsky.social @srouhanifard.bsky.social assess the most abundant RNA modification, pseudouridine, in primary and immortalized human T cells
👇
doi.org/10.1101/2025...
Reposted by Oleksandra Sasha Fanari
Direct RNA sequencing of primary human T cells reveals the impact of immortalization on mRNA pseudouridine modifications. https://www.biorxiv.org/content/10.1101/2025.03.02.641090v1
March 10, 2025 at 5:34 PM
Direct RNA sequencing of primary human T cells reveals the impact of immortalization on mRNA pseudouridine modifications. https://www.biorxiv.org/content/10.1101/2025.03.02.641090v1
Reposted by Oleksandra Sasha Fanari
