Sara McArdle
@saramcardle.bsky.social
Staff Scientist at the La Jolla Institute Microscopy and Histology Facility. I love cat videos and microscopy images and I believe that Matlab is better than Python :)
Took my daughter to one of the San Diego No Kings rallies, because even she knows that you have to stand up and say No to bullies.
October 19, 2025 at 4:53 AM
Took my daughter to one of the San Diego No Kings rallies, because even she knows that you have to stand up and say No to bullies.
All of the segmentation and morphometric analysis was done with the open-source platform QuPath. The scripts I wrote are on Github and the raw images are on the BioImage Archive, because open-source is the way to go!
But seriously, look at these cells! They are so angry.
But seriously, look at these cells! They are so angry.
October 8, 2025 at 5:18 PM
All of the segmentation and morphometric analysis was done with the open-source platform QuPath. The scripts I wrote are on Github and the raw images are on the BioImage Archive, because open-source is the way to go!
But seriously, look at these cells! They are so angry.
But seriously, look at these cells! They are so angry.
We used widefield slidescanning + deconvolution + Z projection to image microglia in the hippocampus. 2 years in with ~11000 cell analyzed and I'm still not over how pretty microglia can be.
We found signs that glia in female AD mice begin to react to *something* before there are even plaques.
We found signs that glia in female AD mice begin to react to *something* before there are even plaques.
October 8, 2025 at 5:18 PM
We used widefield slidescanning + deconvolution + Z projection to image microglia in the hippocampus. 2 years in with ~11000 cell analyzed and I'm still not over how pretty microglia can be.
We found signs that glia in female AD mice begin to react to *something* before there are even plaques.
We found signs that glia in female AD mice begin to react to *something* before there are even plaques.
Happy Fluorescence Friday!
Rainbow Z projection of lymph node follicular dendritic cells.
Rainbow Z projection of lymph node follicular dendritic cells.
September 5, 2025 at 10:44 PM
Happy Fluorescence Friday!
Rainbow Z projection of lymph node follicular dendritic cells.
Rainbow Z projection of lymph node follicular dendritic cells.
I got this in my head and I couldn't focus on work until it existed.
Microscopy image file alignment chart, based on how I feel if you give me an image to analyze:
Microscopy image file alignment chart, based on how I feel if you give me an image to analyze:
August 7, 2025 at 9:27 PM
I got this in my head and I couldn't focus on work until it existed.
Microscopy image file alignment chart, based on how I feel if you give me an image to analyze:
Microscopy image file alignment chart, based on how I feel if you give me an image to analyze:
Registration is OPEN for the 2025 QuPath Training Course on Feb 24-25 and an Advanced Users Meeting on Feb 26 at @lji.org .
The year, the focus will be on Multiplex Immunofluorescence analysis. Hosted by Zbigniew Mikulski, @iamichaelnelson.bsky.social , and myself.
The year, the focus will be on Multiplex Immunofluorescence analysis. Hosted by Zbigniew Mikulski, @iamichaelnelson.bsky.social , and myself.
January 14, 2025 at 12:04 AM
Registration is OPEN for the 2025 QuPath Training Course on Feb 24-25 and an Advanced Users Meeting on Feb 26 at @lji.org .
The year, the focus will be on Multiplex Immunofluorescence analysis. Hosted by Zbigniew Mikulski, @iamichaelnelson.bsky.social , and myself.
The year, the focus will be on Multiplex Immunofluorescence analysis. Hosted by Zbigniew Mikulski, @iamichaelnelson.bsky.social , and myself.
📢Announcing a QuPath Workshop 📢
February 24-25, 2025, in San Diego, CA, at the La Jolla Institute for Immunology.
This year’s event will be all about Multiplex Immunofluorescence Analysis.
The workshop will be followed by an 🎉 Advanced QuPath User’s Meeting 🎉- February 26, 2025.
February 24-25, 2025, in San Diego, CA, at the La Jolla Institute for Immunology.
This year’s event will be all about Multiplex Immunofluorescence Analysis.
The workshop will be followed by an 🎉 Advanced QuPath User’s Meeting 🎉- February 26, 2025.
December 10, 2024 at 6:04 PM
📢Announcing a QuPath Workshop 📢
February 24-25, 2025, in San Diego, CA, at the La Jolla Institute for Immunology.
This year’s event will be all about Multiplex Immunofluorescence Analysis.
The workshop will be followed by an 🎉 Advanced QuPath User’s Meeting 🎉- February 26, 2025.
February 24-25, 2025, in San Diego, CA, at the La Jolla Institute for Immunology.
This year’s event will be all about Multiplex Immunofluorescence Analysis.
The workshop will be followed by an 🎉 Advanced QuPath User’s Meeting 🎉- February 26, 2025.
The world is scary right now, so I'm going to spend today analyzing pretty images and not thinking too hard about what it means that half the country voted for fascism
November 6, 2024 at 5:24 PM
The world is scary right now, so I'm going to spend today analyzing pretty images and not thinking too hard about what it means that half the country voted for fascism
My contribution to Microscopy Monday
September 16, 2024 at 9:47 PM
My contribution to Microscopy Monday
There is still time to register for the #microscopy Multiplex Image Analysis workshop in September in San Diego!
August 28, 2024 at 5:15 PM
There is still time to register for the #microscopy Multiplex Image Analysis workshop in September in San Diego!
I am happily surprised at how well my Thanksgiving desserts came out. Watching a truly embarrassing amount of the Great British Baking Show has paid off!
November 24, 2023 at 3:41 AM
I am happily surprised at how well my Thanksgiving desserts came out. Watching a truly embarrassing amount of the Great British Baking Show has paid off!
This is cool, thank you for sharing! Mine:
September 24, 2023 at 3:13 PM
This is cool, thank you for sharing! Mine:
There is still time to register for the Quantitative BioImaging workshop on Multiplex Image Analysis!
www.quantitativebioimaging.com/qbi2023/mia2...
October 12-13, San Diego
There will be great speakers and interesting round table discussions on the challenges of multiplexing and solutions.
www.quantitativebioimaging.com/qbi2023/mia2...
October 12-13, San Diego
There will be great speakers and interesting round table discussions on the challenges of multiplexing and solutions.
September 13, 2023 at 6:15 PM
There is still time to register for the Quantitative BioImaging workshop on Multiplex Image Analysis!
www.quantitativebioimaging.com/qbi2023/mia2...
October 12-13, San Diego
There will be great speakers and interesting round table discussions on the challenges of multiplexing and solutions.
www.quantitativebioimaging.com/qbi2023/mia2...
October 12-13, San Diego
There will be great speakers and interesting round table discussions on the challenges of multiplexing and solutions.
1 more week to submit an abstract for the Quantitative BioImaging society workshop on Multiplex Image Analysis!
https://www.quantitativebioimaging.com/qbi2023/mia2023/
Hope to see you there!
https://www.quantitativebioimaging.com/qbi2023/mia2023/
Hope to see you there!
August 7, 2023 at 9:43 PM
1 more week to submit an abstract for the Quantitative BioImaging society workshop on Multiplex Image Analysis!
https://www.quantitativebioimaging.com/qbi2023/mia2023/
Hope to see you there!
https://www.quantitativebioimaging.com/qbi2023/mia2023/
Hope to see you there!