Albert Tsai
@rnamods.bsky.social
Reposted by Albert Tsai
Protocol for RNA modification analysis by UHPLC-QqQ MS #STARProtoc www.sciencedirect.com/science/arti...
Protocol for RNA modification analysis by UHPLC-QqQ MS
Over 170 RNA modifications have been reported across domains of life. Here, we present a protocol for identifying and quantifying modified nucleosides…
www.sciencedirect.com
September 14, 2025 at 6:33 AM
Protocol for RNA modification analysis by UHPLC-QqQ MS #STARProtoc www.sciencedirect.com/science/arti...
Reposted by Albert Tsai
Comprehensive nucleoside analysis of archaeal RNA modification profiles reveals an m7G in the conserved P loop of 23S rRNA
Comprehensive nucleoside analysis of archaeal RNA modification profiles reveals an m7G in the conserved P loop of 23S rRNA
Tsai et al. describe comprehensive nucleoside profiles of archaeal RNAs and identify temperature-responsive modifications using LC-MS/MS. They establish a workflow for RNA-modifying enzyme discovery by associating enzyme predictions with global nucleoside profiles. This approach reveals a methyltransferase responsible for installing an m7G in the peptidyl transferase center of 23S rRNA.
dlvr.it
March 25, 2025 at 12:35 PM
Comprehensive nucleoside analysis of archaeal RNA modification profiles reveals an m7G in the conserved P loop of 23S rRNA