Reto Fiolka
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retof.bsky.social
Reto Fiolka
@retof.bsky.social
Optical imaging, interested in STEM topics.
First generation graduate from ETH Zürich
Associate Professor at UT
Southwestern Medical Center
Yes, large fan angles require short focal length lenses.

We made a Plössel lens from two 30mm achromatic doublets.

The short focal length then also results in a tighter lightsheet (for a given aspect ratio).

Light losses are relative. Still better than with massive Gaussian beam expansion.😳
November 5, 2025 at 11:35 PM
How about we discuss details offline? I have a hard time fitting stuff into character limit here.
November 5, 2025 at 10:51 PM
Fan angle matters in the sense that too much angle is hard to pair with existing achromats (and their angular range). We use laser line powell lenses (IIRC 10 and 20 deg).

You indeed can change NA with an adjustable slit. Usually you need to make a relay lens pair to access a Fourier plane.
November 5, 2025 at 8:36 PM
Let us know if you need any more information.

Our system essentially has a fiberlaser collimator (~1mm beam size), then a powell lens in a rotation mount followed by a short focal length (30-50mm, depending on desired NA) achromatic doublet lens.

After that you already have your sheet.
November 4, 2025 at 5:09 PM
We have built all our current lightsheet systems with a Powell lens.

Much more compact set up and more uniform line intensity distribution compared to cylindrical lenses ( for beam expansion and focusing to a sheet).

Only drawback is that the sheet diverges a bit laterally for different colors.
November 4, 2025 at 1:36 AM
L2 is for normies…😅
November 1, 2025 at 10:12 PM
🤣
November 1, 2025 at 10:01 PM
Big if true!
November 1, 2025 at 6:45 AM
New Captcha has just dropped: proof that you are not injective.
November 1, 2025 at 6:45 AM
LLMs are injective; one prompt maps to one output, and true also for other way.

So you can get exact prompt back for any LLM generated blurp.

#SparklyLinearAlgebra
November 1, 2025 at 3:37 AM
Well done James Hayes, Dylan Burnette, Zach Sanchez and Amy Engevik!

Great microscopy and sample preparation skills!
October 27, 2025 at 3:07 PM
Majestic!
October 25, 2025 at 11:29 PM
Yes, that is an iconic dataset.
October 23, 2025 at 4:43 PM
Nice!
October 22, 2025 at 7:28 PM
Haha! Yes, quite a mess…
October 22, 2025 at 3:25 PM
It is a course for the graduate school program.

Kinda the grand math tour.
a man wearing sunglasses is dancing on a bus with his arms outstretched
ALT: a man wearing sunglasses is dancing on a bus with his arms outstretched
media.tenor.com
October 22, 2025 at 2:12 PM