Now clear how much extra information from @nanoporetech.com data - especially full transcripts #nanoporeconf

The reviewer identified a mismatch in the last nucleotide of the 3` side of the forward primer targeting the 12S region, for several species in the order of Gadiformes. The details of that error and its correction are now presented in the supplemental data: onlinelibrary.wiley.com/action/downl...

Getting this published was a long process, and taught us a lot. We initially made an error in the primer design of our new 2kb 12S-16S amplicon. This was fortunately identified by a added new reviewer in the third review round. So all experiments had to be repeated with updated, correct primers...

Ik zag laatst op Planken Wambuis dit donkere dier. Maar die had meer de grootte van de reebok wat het samen mee stond te grazen...

I sequenced total genomic DNA on a previously used flow cell. I got about 2x coverage of the whole genome and >100 fold for the mitogenome.

These are juvenile small-spotted catshark (Scyliorhinus canicula) hatched in August this year. So cool!