Rune Busk Damgaard
@rbdamgaard.bsky.social
Associate Professor, Section for Medical Biotechnology, Technical University of Denmark. Studies ubiquitin signalling in inflammation, metabolism, and disease.
Hope this serves as a useful overview for anyone working on ubiquitin signalling or PTMs. Big thanks to those who did the heavy lifting in the writing process: Julian Koch, Camilla Reiter Elbæk & @dompri.bsky.social 🙌😊
September 16, 2025 at 7:45 AM
Hope this serves as a useful overview for anyone working on ubiquitin signalling or PTMs. Big thanks to those who did the heavy lifting in the writing process: Julian Koch, Camilla Reiter Elbæk & @dompri.bsky.social 🙌😊
We also highlight:
✅ Trade-offs between specificity, throughput, & sensitivity
✅ Gaps in the current toolbox where innovation is needed
✅ The value of combining orthogonal methods for robust insight
✅ Trade-offs between specificity, throughput, & sensitivity
✅ Gaps in the current toolbox where innovation is needed
✅ The value of combining orthogonal methods for robust insight
September 16, 2025 at 7:45 AM
We also highlight:
✅ Trade-offs between specificity, throughput, & sensitivity
✅ Gaps in the current toolbox where innovation is needed
✅ The value of combining orthogonal methods for robust insight
✅ Trade-offs between specificity, throughput, & sensitivity
✅ Gaps in the current toolbox where innovation is needed
✅ The value of combining orthogonal methods for robust insight
…alongside analytical platforms such as:
✅ Immunoblotting 🧫📊
✅ Fluorescence microscopy 🔬🌈
✅ Enzymatic assays 🧪⏱️
✅ Mass spectrometry ⚡📈
✅ Immunoblotting 🧫📊
✅ Fluorescence microscopy 🔬🌈
✅ Enzymatic assays 🧪⏱️
✅ Mass spectrometry ⚡📈
September 16, 2025 at 7:45 AM
…alongside analytical platforms such as:
✅ Immunoblotting 🧫📊
✅ Fluorescence microscopy 🔬🌈
✅ Enzymatic assays 🧪⏱️
✅ Mass spectrometry ⚡📈
✅ Immunoblotting 🧫📊
✅ Fluorescence microscopy 🔬🌈
✅ Enzymatic assays 🧪⏱️
✅ Mass spectrometry ⚡📈
In the review, we summarise state-of-the-art tools for probing linkage-type specificity:
🎯 Antibodies & antibody-like molecules
🎯 Affimers
🎯 Engineered Ub-binding domains
🎯 Catalytically inactive deubiquitinases
🎯 Macrocyclic peptides
🎯 Antibodies & antibody-like molecules
🎯 Affimers
🎯 Engineered Ub-binding domains
🎯 Catalytically inactive deubiquitinases
🎯 Macrocyclic peptides
September 16, 2025 at 7:45 AM
In the review, we summarise state-of-the-art tools for probing linkage-type specificity:
🎯 Antibodies & antibody-like molecules
🎯 Affimers
🎯 Engineered Ub-binding domains
🎯 Catalytically inactive deubiquitinases
🎯 Macrocyclic peptides
🎯 Antibodies & antibody-like molecules
🎯 Affimers
🎯 Engineered Ub-binding domains
🎯 Catalytically inactive deubiquitinases
🎯 Macrocyclic peptides
Why this matters ⬇️
Ubiquitin chain diversity, i.e. different linkage types, mixed or branched chains, is central to cell signalling.
But analysing these chain types specifically remains a big challenge.
Ubiquitin chain diversity, i.e. different linkage types, mixed or branched chains, is central to cell signalling.
But analysing these chain types specifically remains a big challenge.
September 16, 2025 at 7:45 AM
Why this matters ⬇️
Ubiquitin chain diversity, i.e. different linkage types, mixed or branched chains, is central to cell signalling.
But analysing these chain types specifically remains a big challenge.
Ubiquitin chain diversity, i.e. different linkage types, mixed or branched chains, is central to cell signalling.
But analysing these chain types specifically remains a big challenge.
Reposted by Rune Busk Damgaard
the really key number to remember is that biorxiv serves the entire global science community, posting thousands of papers per month for millions of readers, and it's entire running costs ( a few $M/year) are less than some single labs....
July 2, 2025 at 6:27 PM
the really key number to remember is that biorxiv serves the entire global science community, posting thousands of papers per month for millions of readers, and it's entire running costs ( a few $M/year) are less than some single labs....
Our review is published alongside a long list of other excellent reviews in a special issue of ChemBioChem (chemistry-europe.onlinelibrary.wiley.com/doi/toc/10.1... edited by @jnpruneda.bsky.social and Monique Mulder. Thanks a lot for the invitation to contribute to this fantastic issue.
Decoding the Ubiquitin System Using Chemical Biology Tools: ChemBioChem
ChemBioChem is an international journal connecting chemistry and biology, covering chemical biology, biochemistry, synthetic biology, biomaterials & more.
chemistry-europe.onlinelibrary.wiley.com
April 22, 2025 at 11:31 AM
Our review is published alongside a long list of other excellent reviews in a special issue of ChemBioChem (chemistry-europe.onlinelibrary.wiley.com/doi/toc/10.1... edited by @jnpruneda.bsky.social and Monique Mulder. Thanks a lot for the invitation to contribute to this fantastic issue.
Congratulations, Elton and team!
March 18, 2025 at 8:04 AM
Congratulations, Elton and team!