Exploring safer purification strategies is an important step toward protecting both researchers and the environment, while still achieving high-quality results.

As a result, many labs are shifting toward non-toxic alternatives like lysine-based purification and silica-based resins. These methods not only improve lab safety but also help maintain protein integrity and reduce hazardous waste.

The AptaCapture is the future for cell therapy for #rarediseases, and that starts with innovation:
✅ 99% purity in cell isolation (vs. 95%)
✅ 30-minute isolation (vs. 150+ min)
✅ 7.5x cell expansion (vs. 5x)
✅ Lower exhaustion marker (PD1: 32%), improving long-term efficacy #RareDiseaseResearch

Want to know how silica outperforms nickel resins? We’ve got the data.

Check out our latest deep dive on sustainable protein purification. 🌱🔬

www.proteios.com/proteios-blo...

#Biotech #LabSafety #Sustainability #Silica #ProteinPurification

Nickel-based purification harms the planet with heavy metals & hazardous waste.
🌱 Silica is the greener choice:
✅ No toxic nickel—protects ecosystems
✅ No ethanol—reduces VOC emissions
✅ Sustainable disposal—no hazardous waste handling
Purify proteins, not the environment. #Sustainability 3/n

Nickel resins put researchers at risk, ditch the nickel, switch to silica for safer protein purification!

✅ Non-toxic—no heavy metals or health risks
✅ Non-flammable—no ethanol, no fire hazards
✅ Easy disposal—no hazardous waste protocols
Protect your team. Choose safer purification. 2/n

Here’s what we found:
✔ KRAS, TEV protease, and PFK were purified with 85-95% purity.
✔ Yields reached up to 2mg in a single run.
✔ The workflow avoided toxic reagents and took less than 15 minutes.

The gel tells the story—clear, strong bands for some of the toughest-to-purify proteins!
3/3

Here’s what we found:
✔ KRAS, TEV protease, and PFK were purified with 85-95% purity.
✔ Yields reached up to 2mg in a single run.
✔ The workflow avoided toxic reagents and took less than 15 minutes.

The gel tells the story—clear, strong bands for some of the toughest-to-purify proteins!
3/3

To purify we used the RapidPro™ Spin Kit with a streamlined workflow: cell lysis, loading, washing, and elution—all in under 15 minutes.
Each protein faced unique challenges:
🔬 KRAS – medium-low expression in E. coli.
🔬 TEV protease – low solubility.
🔬 PFK – very low expression and yield.
2/n

Hello Will!
Proteios Technology would love to be included in the Seattle Biotech Starter Pack!

✅ Achieved >90% protein purity <30 minutes—directly from crude lysates
✅Gravity columns eliminate the need for pumps or special equipment
✅ Effective for difficult-to-purify proteins, including GFPmut2, mCherry, and MBP, with purities between 80–90%
✅ Hazard-free—no imidazole needed (no toxic waste)

✅ Achieved >90% protein purity <30 minutes—directly from crude lysates
✅Gravity columns eliminate the need for pumps or special equipment
✅ Effective for difficult-to-purify proteins, including GFPmut2, mCherry, and MBP, with purities between 80–90%
✅ Hazard-free—no imidazole needed (no toxic waste)

Read about how the #RapidPro Kits enable purification of low to moderate expressed recombinant proteins. Using the Proteios #Car9 affinity tag, proteins like KRAS, TEV, and PFK were purified in under 15 minutes with 85-95% purity, without toxic metal ions, achieving up to 2mg per column. 5/5