Congratulations to all involved. Raises more questions about all the backup mechanisms in HR. So much to unpack here!

That would be amazing to have! Thanks

ORDERED.......Thanks for the tip!

Yes, yes, yes .......... I want, I need, I drool! Ordering copies for the group tomorrow...

Since this is a native structure, it showcases differential occupancy of ADP and 2-oxoglutarate (2OG) thereby priming the complex for immediate remodeling in response to metabolic demands.

The structure reveals how nitrogenase is inhibited by the NifI complex. Three units of the NifDK tetramer (MoFe protein of nitrogenase) are bundled together by six NifI1a-NifI1b-Nif2 heterotrimers as a 888 kDa supercomplex made of 30 subunits.

Methanogens are dare-devils that operate near the thermodynamic limits of life, conserving significantly less energy during methane production than aerobic diazotrophs. Thus, efficient regulation of nitrogenase is vital for survival under fluctuating nitrogen conditions.

Thanks to funding from the Department of Energy and The Doisy Research Fund @slu-biochemistry.bsky.social

Credit for the structural work goes to @rajnandani.bsky.social @slu-biochemistry.bsky.social Heroic effort from Tom Deere in Dan Lessner's group @uarkpress.bsky.social to isolate the native nitrogenase complex from the methanogen. The structure also shows a novel mode of nitrogenase regulation.

I read it this morning. Wonderful study! Another ssDNA binding protein that is amazing in significance!

Also, check the accompanying article:

Principles of cotranslational mitochondrial protein import: Cell www.cell.com/cell/fulltex...