Even a subtle KD of these transcription factors produced a modest decrease in module 20 that cascaded into substantial composition differences. (12/13)

We validated that at GW16, FEZF2 and TSHZ3 co-express in deep layers of the human cortex, and by GW20, levels of FEZF2 decrease while TSHZ3 expression stays on in these deep layers. (9/13)

However, FEZF2 expression peaks before module 20 activation during development, and half of module 20 genes are candidate targets of FEZF2 (thanks to data from @LodatoS_Lab @ArlottaLab). This included one transcription factor, TSHZ3, linked to autism spectrum disorders. (8/13)

We used these atlases to find gene networks that shape cell types.

Step 1: Define networks w/in each indiv’d in our dataset w/ hierarchical clustering.
Step 2: Correlation analysis btwn indiv’d to id which networks co-expressed across the entire meta-atlas – these are our meta-modules. (4/13)