@PoratShliomLab
@poratshliomlab.bsky.social
Cell Biologist and microscopist. My lab
@NCIResearchCtr focuses on mitochondria, metabolism, and liver cancer. Views are my own #cellbiology #microscopy
@NCIResearchCtr focuses on mitochondria, metabolism, and liver cancer. Views are my own #cellbiology #microscopy
7. Our model: in homeostasis, two types of mitochondria operate simultaneously: periportal mitochondria with a round morphology and a preference for fatty acid oxidation and pericentral mitochondria that synthesize TGs and store them through contact with LDs. more coming...
December 12, 2024 at 5:15 PM
7. Our model: in homeostasis, two types of mitochondria operate simultaneously: periportal mitochondria with a round morphology and a preference for fatty acid oxidation and pericentral mitochondria that synthesize TGs and store them through contact with LDs. more coming...
6. Can introducing mito-LD contacts in WD reduce toxicity? YES! OE of PLIN5 increased mito-LD interactions, improved redox status, and reduced steatosis—highlighting the physiological significance of organelle interactions in lipid homeostasis and tissue health.
December 12, 2024 at 5:15 PM
6. Can introducing mito-LD contacts in WD reduce toxicity? YES! OE of PLIN5 increased mito-LD interactions, improved redox status, and reduced steatosis—highlighting the physiological significance of organelle interactions in lipid homeostasis and tissue health.
5. How do mito-LD contacts regulate lipids? we overexpressed PLIN5 variants in vivo (thanks to @cohenlaboratory.bsky.social for the constructs). We found that 1. PLIN5-induced mito-LD contacts are phosphorylation-dependent. 2. Mito-LD contacts promote expansion and triglyceride synthesis
December 12, 2024 at 5:14 PM
5. How do mito-LD contacts regulate lipids? we overexpressed PLIN5 variants in vivo (thanks to @cohenlaboratory.bsky.social for the constructs). We found that 1. PLIN5-induced mito-LD contacts are phosphorylation-dependent. 2. Mito-LD contacts promote expansion and triglyceride synthesis
4. We performed spatial proteomics on sorted hepatocytes to identify the proteins that mediate mitochondria-LD contacts. Many of these proteins are upregulated by fasting, with PLIN5 at the top of the list.
December 12, 2024 at 5:13 PM
4. We performed spatial proteomics on sorted hepatocytes to identify the proteins that mediate mitochondria-LD contacts. Many of these proteins are upregulated by fasting, with PLIN5 at the top of the list.
3. One of the significant phenotypes we observed was in fasted mice, where mitochondria-LD contacts were abundant throughout the lobule. In WD-fed mice, however, those interactions were rare.
December 12, 2024 at 5:13 PM
3. One of the significant phenotypes we observed was in fasted mice, where mitochondria-LD contacts were abundant throughout the lobule. In WD-fed mice, however, those interactions were rare.
2. Check out how organelle features change across space and diet! This is just like gene expression signatures! Plus, it can be used for hepatocyte clustering in PCA 😎😎😎
December 12, 2024 at 5:12 PM
2. Check out how organelle features change across space and diet! This is just like gene expression signatures! Plus, it can be used for hepatocyte clustering in PCA 😎😎😎
1. We discovered that the spatial positioning of hepatocytes and dietary intervention influenced mitochondrial and LD features. This supports the notion that organelle features encode signatures of zonation and can provide insights into the metabolic state of hepatocytes.
December 12, 2024 at 5:12 PM
1. We discovered that the spatial positioning of hepatocytes and dietary intervention influenced mitochondrial and LD features. This supports the notion that organelle features encode signatures of zonation and can provide insights into the metabolic state of hepatocytes.
Preprint alert!! How zonated hepatocytes adapt to changes in lipid influx due to fasting or a Western diet (WD). We developed a microscopy-based phenotyping method called single-cell phenomics (scPhenomics) to measure the features of mitochondria and lipid droplets (LDs).
December 12, 2024 at 5:11 PM
Preprint alert!! How zonated hepatocytes adapt to changes in lipid influx due to fasting or a Western diet (WD). We developed a microscopy-based phenotyping method called single-cell phenomics (scPhenomics) to measure the features of mitochondria and lipid droplets (LDs).
Our annual lab-giving celebration! I am grateful to have such an energetic and positive team!
Happy holiday season from the Porat-Shliom lab🎄🕎
@kbarrows.bsky.social @ivyxiong.bsky.social
Happy holiday season from the Porat-Shliom lab🎄🕎
@kbarrows.bsky.social @ivyxiong.bsky.social
December 11, 2024 at 7:26 PM
Our annual lab-giving celebration! I am grateful to have such an energetic and positive team!
Happy holiday season from the Porat-Shliom lab🎄🕎
@kbarrows.bsky.social @ivyxiong.bsky.social
Happy holiday season from the Porat-Shliom lab🎄🕎
@kbarrows.bsky.social @ivyxiong.bsky.social
I was pleased to present our work @umncbs, and interact with exceptional graduate students and faculty. Thank you @dgmashek, for the invitation
December 9, 2024 at 10:12 PM
I was pleased to present our work @umncbs, and interact with exceptional graduate students and faculty. Thank you @dgmashek, for the invitation
This summer, traveling to Israel was far from trivial. I’m grateful for being able to reconnect with family and friends, celebrate my bday and recharge. In the pic, hopeful sunset captured in Tel Aviv.
December 9, 2024 at 10:12 PM
This summer, traveling to Israel was far from trivial. I’m grateful for being able to reconnect with family and friends, celebrate my bday and recharge. In the pic, hopeful sunset captured in Tel Aviv.
Saying goodbye is never easy, this time, it is especially hard. After 5 wonderful years, @SunWooSophieKa1 is heading back to Australia. Grateful for the opportunity to work with you. We will miss you!!
December 9, 2024 at 10:12 PM
Saying goodbye is never easy, this time, it is especially hard. After 5 wonderful years, @SunWooSophieKa1 is heading back to Australia. Grateful for the opportunity to work with you. We will miss you!!
Saying good bye to Colin Miller who is heading to medical school. It was wonderful working together and we are all very excited for you!
December 9, 2024 at 10:12 PM
Saying good bye to Colin Miller who is heading to medical school. It was wonderful working together and we are all very excited for you!
I’m not the only one, staring at the sun 😊
December 9, 2024 at 10:11 PM
I’m not the only one, staring at the sun 😊
Did you know that hepatocytes, located 300 microns apart, have different mitochondria? Learn how we use advanced microscopy to study mitochondrial physiology in vivo. Sunday 8:45 am Conv. Ctr., 102 #APS2024 #liverzonation #mitochondria
December 9, 2024 at 10:11 PM
Did you know that hepatocytes, located 300 microns apart, have different mitochondria? Learn how we use advanced microscopy to study mitochondrial physiology in vivo. Sunday 8:45 am Conv. Ctr., 102 #APS2024 #liverzonation #mitochondria
It was a pleasure hosting @labs_mann to discuss the latest advancements in single-cell proteomics and applications to understanding human diseases.
@NCIResearchCtr @IRPatNIH
@NCIResearchCtr @IRPatNIH
December 9, 2024 at 10:11 PM
It was a pleasure hosting @labs_mann to discuss the latest advancements in single-cell proteomics and applications to understanding human diseases.
@NCIResearchCtr @IRPatNIH
@NCIResearchCtr @IRPatNIH
There's nothing quite like the energy of curious minds joining forces to tackle a challenging question!
And sharing food afterward? An added bonus 🙃
@NCIResearchCtr @IRPatNIH #teamscience #cellbiology #liverphysiology
And sharing food afterward? An added bonus 🙃
@NCIResearchCtr @IRPatNIH #teamscience #cellbiology #liverphysiology
December 9, 2024 at 10:11 PM
There's nothing quite like the energy of curious minds joining forces to tackle a challenging question!
And sharing food afterward? An added bonus 🙃
@NCIResearchCtr @IRPatNIH #teamscience #cellbiology #liverphysiology
And sharing food afterward? An added bonus 🙃
@NCIResearchCtr @IRPatNIH #teamscience #cellbiology #liverphysiology
Looking forward to presenting our latest on mitochondria zonation at the Bioenergetics and Mitochondrial Biophysics session in Philly, Sunday 4-6 pm, along with an amazing lineup of speakers.
#bps2024 @BiophysicalSoc
#bps2024 @BiophysicalSoc
December 9, 2024 at 10:11 PM
Looking forward to presenting our latest on mitochondria zonation at the Bioenergetics and Mitochondrial Biophysics session in Philly, Sunday 4-6 pm, along with an amazing lineup of speakers.
#bps2024 @BiophysicalSoc
#bps2024 @BiophysicalSoc
Happy to share my recent Current Opinion in Cell Biology @ELS_CellBiology
What is liver zonation? How do hepatocytes organize their activities in the tissue? And what does cell-cell communication and gradients have to do with it?
authors.elsevier.com/a/1iD9i3PA3sV0…
What is liver zonation? How do hepatocytes organize their activities in the tissue? And what does cell-cell communication and gradients have to do with it?
authors.elsevier.com/a/1iD9i3PA3sV0…
December 9, 2024 at 10:11 PM
Happy to share my recent Current Opinion in Cell Biology @ELS_CellBiology
What is liver zonation? How do hepatocytes organize their activities in the tissue? And what does cell-cell communication and gradients have to do with it?
authors.elsevier.com/a/1iD9i3PA3sV0…
What is liver zonation? How do hepatocytes organize their activities in the tissue? And what does cell-cell communication and gradients have to do with it?
authors.elsevier.com/a/1iD9i3PA3sV0…
Looking forward to learning about everything new and exciting in cell biology #cellbio2023
Come hear about mitochondria zonation in the liver
Come hear about mitochondria zonation in the liver
December 9, 2024 at 10:10 PM
Looking forward to learning about everything new and exciting in cell biology #cellbio2023
Come hear about mitochondria zonation in the liver
Come hear about mitochondria zonation in the liver
Congratulations to @SunWooSophieKa1, Lauryn Brown and Colin Miler for presenting their work at the CCR fellows and young investigators and to Colin for receiving the outstanding postgraduate award!! Fantastic day of science @NCIResearchCtr @NCI_Training
December 9, 2024 at 10:10 PM
Congratulations to @SunWooSophieKa1, Lauryn Brown and Colin Miler for presenting their work at the CCR fellows and young investigators and to Colin for receiving the outstanding postgraduate award!! Fantastic day of science @NCIResearchCtr @NCI_Training
We have a postdoc position available! DM me or come to meet virtually on May 10 at 3:30 at the NIH Postdoc Recruiting event (link below). Please RT 🙏
December 9, 2024 at 10:10 PM
We have a postdoc position available! DM me or come to meet virtually on May 10 at 3:30 at the NIH Postdoc Recruiting event (link below). Please RT 🙏
The liver is a work of art
December 9, 2024 at 10:09 PM
The liver is a work of art
Did I already said that the best thing @ASCBiology #Cellbio2022 is getting together with good friends?!
@LabFriedman @LLLackner @HenneLab @narayan_pri
@LabFriedman @LLLackner @HenneLab @narayan_pri
December 9, 2024 at 10:09 PM
Did I already said that the best thing @ASCBiology #Cellbio2022 is getting together with good friends?!
@LabFriedman @LLLackner @HenneLab @narayan_pri
@LabFriedman @LLLackner @HenneLab @narayan_pri
The BEST part of @ASCBiology #CellBio2022 is getting together with good friends @sehamebrahim
December 9, 2024 at 10:09 PM
The BEST part of @ASCBiology #CellBio2022 is getting together with good friends @sehamebrahim