Shreeram 'Ram' Akilesh
@podocytes.bsky.social
Associate Professor, Renal Pathologist and Kidney Genomics/Spatial -omics Researcher. Posts represent my opinions not medical advice. #renalpath #NephSky.
http://faculty.washington.edu/shreeram/
http://faculty.washington.edu/shreeram/
Our latest work studying the reproducibility and sensitivity tradeoffs in spatial transcriptomics. All done with clinically sourced tissues as a foundation for future diagnostics - collab with @kelsmithpath.bsky.social : doi.org/10.1016/j.la...
Redirecting
doi.org
April 30, 2025 at 6:38 PM
Our latest work studying the reproducibility and sensitivity tradeoffs in spatial transcriptomics. All done with clinically sourced tissues as a foundation for future diagnostics - collab with @kelsmithpath.bsky.social : doi.org/10.1016/j.la...
We learn a lot from our patient's kidney biopsies. In collaboration with Astrid Weins (Brigham), we identified 2 patients with defects in peripheral immune tolerance (no Tregs, ICI tx for cancer). Both developed anti-nephrin antibody-associated minimal change disease: karger.com/gdz/article/...
Genetic and Iatrogenic Defects in Peripheral Tolerance Associated with Anti-Nephrin Antibody-Associated Minimal Change Disease
Abstract. Introduction: Minimal change disease (MCD) is a common cause of nephrotic syndrome in children and adults. Immune dysregulation is a contributor, but the relative roles of individual compone...
karger.com
February 25, 2025 at 3:12 PM
We learn a lot from our patient's kidney biopsies. In collaboration with Astrid Weins (Brigham), we identified 2 patients with defects in peripheral immune tolerance (no Tregs, ICI tx for cancer). Both developed anti-nephrin antibody-associated minimal change disease: karger.com/gdz/article/...
A little mosaic project of my favorite cell 🐙
January 27, 2025 at 3:15 AM
A little mosaic project of my favorite cell 🐙
Oooh pretty!! Many thanks to @susanallison.bsky.social and the entire staff of @natrevneph.bsky.social for helping @oligopain.bsky.social and me with this review. LOVE ❤️ the cover!!
Our cover for the February issue is inspired by our 'Guide-to' article on approaches to study the 3D and 4D genome in the kidney, written by @podocytes.bsky.social and @oligopain.bsky.social. Read it here: go.nature.com/4jvhZvo
January 25, 2025 at 3:38 PM
Oooh pretty!! Many thanks to @susanallison.bsky.social and the entire staff of @natrevneph.bsky.social for helping @oligopain.bsky.social and me with this review. LOVE ❤️ the cover!!
Reposted by Shreeram 'Ram' Akilesh
Delighted to share our latest paper:
"The glomerular circadian clock temporally regulates basement membrane dynamics and the podocyte glucocorticoid response"
Expertly led by Rebecca Preston & in collaboration with clock superstar Qing Jun Meng
pubmed.ncbi.nlm.nih.gov/39515644/
Key findings 👇👇
November 21, 2024 at 3:39 PM
Delighted to share our latest paper:
"The glomerular circadian clock temporally regulates basement membrane dynamics and the podocyte glucocorticoid response"
Expertly led by Rebecca Preston & in collaboration with clock superstar Qing Jun Meng
pubmed.ncbi.nlm.nih.gov/39515644/
Key findings 👇👇
Somewhere over the kidney rainbow... 🫘🌈 #NephSky #renalpath
November 21, 2024 at 12:33 AM
Somewhere over the kidney rainbow... 🫘🌈 #NephSky #renalpath
Reposted by Shreeram 'Ram' Akilesh
The pSABER paper is now online!
pSABER is a new method for amplifying in situ hybridization signals
This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social
doi.org/10.1038/s415...
🧵 1/12
pSABER is a new method for amplifying in situ hybridization signals
This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social
doi.org/10.1038/s415...
🧵 1/12
Efficient and highly amplified imaging of nucleic acid targets in cellular and histopathological samples with pSABER - Nature Methods
pSABER combines the power of signal amplification by exchange reaction (SABER) with the deposition of fluorescent or colorimetric substrates by horseradish peroxidase to enable enhanced signals for in...
doi.org
November 15, 2024 at 10:27 PM
The pSABER paper is now online!
pSABER is a new method for amplifying in situ hybridization signals
This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social
doi.org/10.1038/s415...
🧵 1/12
pSABER is a new method for amplifying in situ hybridization signals
This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social
doi.org/10.1038/s415...
🧵 1/12