Penny
@penny-w.bsky.social
Reposted by Penny
Di-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets!
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
Covalently constrained ‘Di-Gembodies’ enable parallel structure solutions by cryo-EM - Nature Chemical Biology
Disulfide-based dimerization of modified identical and heterologous nanobody scaffolds enables higher-order assembly for high-resolution cryo-electron microscopy structure determination that is widely...
www.nature.com
August 15, 2025 at 10:00 AM
Di-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets!
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social
Out now in Nature Chemical Biology @natchembio.nature.com
www.nature.com/articles/s41...
Reposted by Penny
Great work highlighting how important it is to carry out experimental structure determination instead relying on AlphaFold predictions. #crystallography #structure #biology #chemistry
www.nature.com/articles/s41...
www.nature.com/articles/s41...
AlphaFold predictions are valuable hypotheses and accelerate but do not replace experimental structure determination - Nature Methods
An analysis of AlphaFold protein structure predictions shows that while in many cases the predictions are highly accurate, there are also many instances where the predicted structures or parts of pred...
www.nature.com
May 25, 2025 at 5:54 PM
Great work highlighting how important it is to carry out experimental structure determination instead relying on AlphaFold predictions. #crystallography #structure #biology #chemistry
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Penny
Check out our latest preprint with the Qi lab on the structure and disease physiology of the human ERAD complex. Outstanding work by Leo, a cell physiologist whom we successfully tempted into structural biology! Preprint: www.biorxiv.org/content/10.1...
June 16, 2025 at 2:42 PM
Check out our latest preprint with the Qi lab on the structure and disease physiology of the human ERAD complex. Outstanding work by Leo, a cell physiologist whom we successfully tempted into structural biology! Preprint: www.biorxiv.org/content/10.1...
Reposted by Penny
Theme 1 of the embo.org ESCRT meeting: Tentacles across scales 🐙 …from simulated asgard archaea to swirly filaments 🌀
Andela Saric, Adai Colom, Qing-Tao Shen, John McCullough 🧶🧬🧪
Andela Saric, Adai Colom, Qing-Tao Shen, John McCullough 🧶🧬🧪
June 27, 2025 at 6:40 PM
Theme 1 of the embo.org ESCRT meeting: Tentacles across scales 🐙 …from simulated asgard archaea to swirly filaments 🌀
Andela Saric, Adai Colom, Qing-Tao Shen, John McCullough 🧶🧬🧪
Andela Saric, Adai Colom, Qing-Tao Shen, John McCullough 🧶🧬🧪
Reposted by Penny
Einenkel et al 𝘕𝘢𝘵 𝘔𝘪𝘤𝘳𝘰 🦠
Cryo-EM structures of the complete 𝘚𝘢𝘭𝘮𝘰𝘯𝘦𝘭𝘭𝘢 extracellular flagellum—including the FliD cap complex and hook–filament junction. Flagellin subunits assemble while the junction buffers mechanical stress → stable motility and colonization.
www.nature.com/articles/s41...
Cryo-EM structures of the complete 𝘚𝘢𝘭𝘮𝘰𝘯𝘦𝘭𝘭𝘢 extracellular flagellum—including the FliD cap complex and hook–filament junction. Flagellin subunits assemble while the junction buffers mechanical stress → stable motility and colonization.
www.nature.com/articles/s41...
July 2, 2025 at 12:55 AM
Einenkel et al 𝘕𝘢𝘵 𝘔𝘪𝘤𝘳𝘰 🦠
Cryo-EM structures of the complete 𝘚𝘢𝘭𝘮𝘰𝘯𝘦𝘭𝘭𝘢 extracellular flagellum—including the FliD cap complex and hook–filament junction. Flagellin subunits assemble while the junction buffers mechanical stress → stable motility and colonization.
www.nature.com/articles/s41...
Cryo-EM structures of the complete 𝘚𝘢𝘭𝘮𝘰𝘯𝘦𝘭𝘭𝘢 extracellular flagellum—including the FliD cap complex and hook–filament junction. Flagellin subunits assemble while the junction buffers mechanical stress → stable motility and colonization.
www.nature.com/articles/s41...
Reposted by Penny
🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
April 25, 2025 at 7:28 AM
🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
Reposted by Penny
Let’s average all the things! Visual proteomics time?😉 #TeamTomo 🧪🧶🧬🔬
To all #ChlamyDataset enthusiasts, here is a script that lets you directly import the pre-processed results from EMPIAR-11830 into #RELION v5 for subtomogram averaging:
github.com/Chromatin-St...
#Chlamydomonas #TeamTomo #CryoET #OpenSoftwareAcceleratesScience
github.com/Chromatin-St...
#Chlamydomonas #TeamTomo #CryoET #OpenSoftwareAcceleratesScience
ChlamyAnnotations/chlamydataset2relion5 at master · Chromatin-Structure-Rhythms-Lab/ChlamyAnnotations
Particle annotations for the large-scale cryo-ET dataset of Chlamydomonas reinhardtii - Chromatin-Structure-Rhythms-Lab/ChlamyAnnotations
github.com
April 28, 2025 at 4:40 PM
Let’s average all the things! Visual proteomics time?😉 #TeamTomo 🧪🧶🧬🔬
Reposted by Penny
Aviadenovirus structure: a highly thermostable capsid in the absence of stabilizing proteins https://www.biorxiv.org/content/10.1101/2025.04.23.650237v1
April 24, 2025 at 3:17 AM
Aviadenovirus structure: a highly thermostable capsid in the absence of stabilizing proteins https://www.biorxiv.org/content/10.1101/2025.04.23.650237v1
Reposted by Penny
One of the largest open questions in anaerobic metabolism solved. The best work of my lab yet, performed by the incredible hard working, talented and motivated coworkers: @fidelormz.bsky.social , @sophia-pa.bsky.social and @rnfr2d2.bsky.social. I am a proud PI today!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structure of the ATP-driven methyl-coenzyme M reductase activation complex - Nature
The structure and function of the MCR activation complex from Methanococcus maripaludis were revealed, demonstrating its ATP-dependent ability to activate MCR and form methane while uncovering a uniqu...
www.nature.com
April 16, 2025 at 4:09 PM
One of the largest open questions in anaerobic metabolism solved. The best work of my lab yet, performed by the incredible hard working, talented and motivated coworkers: @fidelormz.bsky.social , @sophia-pa.bsky.social and @rnfr2d2.bsky.social. I am a proud PI today!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Penny
Some eye-catching #TeamTomo work here. Congrats to @kfreeman.bsky.social and all the authors! 🧪🧶🧬🔬
Krista Freeman et al 𝘊𝘌𝘓𝘓
stunning atomic-level imaging (Cryo-EM, cryo-ET) reveals how 𝐛𝐚𝐜𝐭𝐞𝐫𝐢𝐨𝐩𝐡𝐚𝐠𝐞 Bxb1 reshapes its tail tip to breach the mycobacterial cell wall and deliver DNA to the cytoplasm
can inform precise targeted phage therapies for TB and NTM infections
www.cell.com/cell/fulltex...
stunning atomic-level imaging (Cryo-EM, cryo-ET) reveals how 𝐛𝐚𝐜𝐭𝐞𝐫𝐢𝐨𝐩𝐡𝐚𝐠𝐞 Bxb1 reshapes its tail tip to breach the mycobacterial cell wall and deliver DNA to the cytoplasm
can inform precise targeted phage therapies for TB and NTM infections
www.cell.com/cell/fulltex...
April 18, 2025 at 7:55 PM
Some eye-catching #TeamTomo work here. Congrats to @kfreeman.bsky.social and all the authors! 🧪🧶🧬🔬