Paul Bollyky
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pbollyky.bsky.social
Paul Bollyky
@pbollyky.bsky.social
Stanford University Infectious Disease Doc studying bacteriophages, bacterial infections, and innate immunity.
We find JukA (formerly YaaW, WP_003137196.1) in 10 of our 80 CPA. With OMK01, The 10 Juk+ strains are resistant but so are 66 Juk- strains. The 4 susceptible strains are indeed Juk-. The association with Juk was non-significant.
December 11, 2024 at 6:29 AM
Thanks for these great questions Chase and for your interest in our work!
December 6, 2024 at 10:02 PM
In a recent pre-print, we used transposon mutagenic to identify the receptors for OMK01 and find that it indeed used flagella www.biorxiv.org/content/10.1...
Bacterial Receptors but Not Anti-Phage Defence Mechanisms Determine Host Range for a Pair of Pseudomonas aeruginosa Lytic Phages
Lytic bacteriophages (phage), viral predators of bacteria, offer a promising solution for treating bacterial infections. However, the narrow host range of phages limits the broad utility of individual...
www.biorxiv.org
December 6, 2024 at 10:01 PM
With closely related phages having distinct receptor usage patterns, we address this in figure 2 - we find that this is the case for several phages, suggesting that phages may switch receptors with some frequency.
Bacterial Receptors but Not Anti-Phage Defence Mechanisms Determine Host Range for a Pair of Pseudomonas aeruginosa Lytic Phages
Lytic bacteriophages (phage), viral predators of bacteria, offer a promising solution for treating bacterial infections. However, the narrow host range of phages limits the broad utility of individual...
www.biorxiv.org
December 6, 2024 at 10:00 PM
I would wonder what MOI was used in other studies with phikz and PA14. We used 100.
December 6, 2024 at 10:00 PM
Thank you!!
December 4, 2024 at 1:26 AM
Many thanks to our outstanding collaborators including Liz Burgener, Carlos Milla, and Niaz Banaei
December 2, 2024 at 6:40 AM
In this work, we describe a streamlined protocol for identifying cross-resistant phages and antibiotics, which we call complementarity grouping. Cocktails targeting 3 or more complementarity groups were effective vs >80% of P. aeruginosa isolates. Add a synergistic antibiotic and its >96%
December 2, 2024 at 6:39 AM