Paul McLaren
@paulmclaren.bsky.social
Now a sensible man, by and by a fool, and presently a beast.
Oddly enough we didn't see the same effect in a T cell line (Jurkats), suggesting it might be a cell type (or cell state) specific effect
November 21, 2023 at 9:41 PM
Oddly enough we didn't see the same effect in a T cell line (Jurkats), suggesting it might be a cell type (or cell state) specific effect
We saw a similar thing in monocytic THP1 cell lines (ED figure 4) and in iPSC-derived macrophages (where less CHD1L meant more HIV)
November 21, 2023 at 9:40 PM
We saw a similar thing in monocytic THP1 cell lines (ED figure 4) and in iPSC-derived macrophages (where less CHD1L meant more HIV)
To try to get to the bottom of that, we used #CRISPR to knock out CHD1L in a model cell line (U2OS) and saw a pretty big jump in HIV infection in KO cells. An effect that was reduced when we added CHD1L back
November 21, 2023 at 9:39 PM
To try to get to the bottom of that, we used #CRISPR to knock out CHD1L in a model cell line (U2OS) and saw a pretty big jump in HIV infection in KO cells. An effect that was reduced when we added CHD1L back
Anyhow, why didn't we find this in earlier (larger) studies in people living with HIV of European ancestry? pnas.org/doi/10.1073/... Because the associated variant(s) aren't present in non-African ancestry populations!
November 21, 2023 at 9:38 PM
Anyhow, why didn't we find this in earlier (larger) studies in people living with HIV of European ancestry? pnas.org/doi/10.1073/... Because the associated variant(s) aren't present in non-African ancestry populations!
