Oh sorry, I am at MSU in the Secor lab.

Any in-person slots ?

Congrats!!! That's so awesome 🚀

Thank you!

Thank you. So nice to hear from you!

Phollow provides a platform to unravel the hidden lives of phages, capturing their nanoscopic dynamics within microscopic bacterial communities and macroscopic animal hosts. Check all the details in our manuscript!

We also found that antibiotics induce phage outbreaks and viral transmission that ultimately remodels the composition of gut microbial communities.

and more strikingly, virions disseminating to extraintestinal tissues including the liver and brain. magenta = DNA, white= phage particles.

phages (in white) being internalized by gut-lining cells, including enteroendocrine cells (in green). pink = nuclei of intestinal tissues.

virions closely associating with the intestinal mucosa and tissue...

The following videos reveals the aftermath of a phage outbreak: virions dispersed throughout the lumen...

We then brought Phollow into the gut of live zebrafish, taking advantage of their transparency and small size to visualize microbial communities across the entire intestine —capturing their dynamic interactions with the host in real time! pink = DNA, purple = actin, green = E. coli

Phollow enables tracking chains of interbacterial transmission. As phages infect new bacterial hosts, they’re re-tagged with a new fluorescent color. Here, green virions from green-labeled cells infect pink- and cyan-labeled targets, generating vibrant confetti bursts of differently colored phages.

One of my favorite experiments with Phollow are time lapses that allow us to illuminate the secret lives of phages. Here, we captured P2 temperate phages undergoing lytic replication in E. coli, leading to explosive host cell lysis and rapid virion dispersal into the environment.

Congrats!