Brian Beliveau
@oligopain.bsky.social
PI @ UW Genome Sciences | chromosome biology | 3D genome | chromatin | single cell & spatial biology
We develop technologies to study genomes and the things that are made from them. Avid FISHer.
beliveau.io
We develop technologies to study genomes and the things that are made from them. Avid FISHer.
beliveau.io
If any 🔬 peeps want a distraction from doomscrolling through the current hellscape, here is a puzzle we just uncovered:
Top row of image seems to be from the bottom of the exposed area on the camera chip, then everything shifts down
Happens full frame and with ROI. Nikon Elements, Sona sCMOs
????
Top row of image seems to be from the bottom of the exposed area on the camera chip, then everything shifts down
Happens full frame and with ROI. Nikon Elements, Sona sCMOs
????
March 12, 2025 at 7:37 PM
If any 🔬 peeps want a distraction from doomscrolling through the current hellscape, here is a puzzle we just uncovered:
Top row of image seems to be from the bottom of the exposed area on the camera chip, then everything shifts down
Happens full frame and with ROI. Nikon Elements, Sona sCMOs
????
Top row of image seems to be from the bottom of the exposed area on the camera chip, then everything shifts down
Happens full frame and with ROI. Nikon Elements, Sona sCMOs
????
Standing up for science in Seattle
March 7, 2025 at 8:40 PM
Standing up for science in Seattle
Surprised it isn't higher, given everything
February 14, 2025 at 1:44 AM
Surprised it isn't higher, given everything
One of the hidden gems in my lab these days is a codebase to pull down our roster from the lab website and auto-gen a figure for acknowledgements slides. We use it so often.
Would this be broadly useful? LMK ... we could try to publish and support.
Would this be broadly useful? LMK ... we could try to publish and support.
February 13, 2025 at 6:09 AM
One of the hidden gems in my lab these days is a codebase to pull down our roster from the lab website and auto-gen a figure for acknowledgements slides. We use it so often.
Would this be broadly useful? LMK ... we could try to publish and support.
Would this be broadly useful? LMK ... we could try to publish and support.
Is this my lab's way of telling me I should be in the office more 🤔😂
January 22, 2025 at 6:02 PM
Is this my lab's way of telling me I should be in the office more 🤔😂
Upping my commute -> office experience, should have done this years ago
January 10, 2025 at 7:22 PM
Upping my commute -> office experience, should have done this years ago
Happy holidays, from our mise to yours 🥘
December 24, 2024 at 11:53 PM
Happy holidays, from our mise to yours 🥘
There are many great things about working at @UWGenome. This may be the greatest.
Been going for >2 years now! 🐱🗓️
Been going for >2 years now! 🐱🗓️
November 20, 2024 at 7:51 PM
There are many great things about working at @UWGenome. This may be the greatest.
Been going for >2 years now! 🐱🗓️
Been going for >2 years now! 🐱🗓️
The most stressful channel on the workspace 🟨⬛🟩⬛⬛
November 19, 2024 at 7:04 PM
The most stressful channel on the workspace 🟨⬛🟩⬛⬛
I can think of worse ways to spend a Friday night 🍕
November 16, 2024 at 3:19 AM
I can think of worse ways to spend a Friday night 🍕
Finally, a big benefit of pSABER being super bright is that you can readily image it on low NA, air immersion objectives. SABER is essentially undetectable on these systems.
We are really excited about pSABER for applications like optical screening
8/12
We are really excited about pSABER for applications like optical screening
8/12
November 15, 2024 at 10:27 PM
Finally, a big benefit of pSABER being super bright is that you can readily image it on low NA, air immersion objectives. SABER is essentially undetectable on these systems.
We are really excited about pSABER for applications like optical screening
8/12
We are really excited about pSABER for applications like optical screening
8/12
I was lucky enough to image another exchange-pSABER experiment carried out by Sahar targeting three RNA species on our SoRa confocal.
This might be my favorite image ever taken.
As a microscopist, it is hard to put into words just how incredible i is to see this on the scope itself.
7/12
This might be my favorite image ever taken.
As a microscopist, it is hard to put into words just how incredible i is to see this on the scope itself.
7/12
November 15, 2024 at 10:27 PM
I was lucky enough to image another exchange-pSABER experiment carried out by Sahar targeting three RNA species on our SoRa confocal.
This might be my favorite image ever taken.
As a microscopist, it is hard to put into words just how incredible i is to see this on the scope itself.
7/12
This might be my favorite image ever taken.
As a microscopist, it is hard to put into words just how incredible i is to see this on the scope itself.
7/12
We used DNA-exchange to label mouse minor satellite (DNA pSABER), 7SK (RNA pSABER), and the aquaporin protein (conventional IF) in FFPE sections.
The resulting images that Sahar acquired are simply gorgeous
6/12
The resulting images that Sahar acquired are simply gorgeous
6/12
November 15, 2024 at 10:27 PM
We used DNA-exchange to label mouse minor satellite (DNA pSABER), 7SK (RNA pSABER), and the aquaporin protein (conventional IF) in FFPE sections.
The resulting images that Sahar acquired are simply gorgeous
6/12
The resulting images that Sahar acquired are simply gorgeous
6/12
Importantly, because pSABER simply layers more signal amplification onto SABER, we can harness all the benefits inherent to SABER as well.
E.g., we can still use DNA-exchange to multiplex
5/12
E.g., we can still use DNA-exchange to multiplex
5/12
November 15, 2024 at 10:27 PM
Importantly, because pSABER simply layers more signal amplification onto SABER, we can harness all the benefits inherent to SABER as well.
E.g., we can still use DNA-exchange to multiplex
5/12
E.g., we can still use DNA-exchange to multiplex
5/12
Happy #FluorescenceFriday!
Here are some HCT116 nuclei with some ... "friends" ... imaged on our SoRa confocal
Gave us quite the shock when we first saw it 😅
Here are some HCT116 nuclei with some ... "friends" ... imaged on our SoRa confocal
Gave us quite the shock when we first saw it 😅
November 15, 2024 at 7:44 PM
Happy #FluorescenceFriday!
Here are some HCT116 nuclei with some ... "friends" ... imaged on our SoRa confocal
Gave us quite the shock when we first saw it 😅
Here are some HCT116 nuclei with some ... "friends" ... imaged on our SoRa confocal
Gave us quite the shock when we first saw it 😅
🎶 I'm not always there when you call...
...but I'm always on time 🎶
...but I'm always on time 🎶
November 15, 2024 at 7:07 PM
🎶 I'm not always there when you call...
...but I'm always on time 🎶
...but I'm always on time 🎶
Glad to see the lab is making the most of freezer defrost day ☃️
October 5, 2023 at 9:54 PM
Glad to see the lab is making the most of freezer defrost day ☃️