Nogales Lab
@nogaleslab.bsky.social
The Nogales Lab @ UC Berkeley. #CryoEM #Transcription #Microtubules #ChromatinModification Bluesky account managed by lab members.
Delighted to present my trajectory and research to audiences ranging from high school students to researchers at Hong Kong Universities and Science Centers. Special thanks to my local ambassadors Zhoe and Emi for making my visit so special 🥰🥰
November 14, 2025 at 3:24 AM
Delighted to present my trajectory and research to audiences ranging from high school students to researchers at Hong Kong Universities and Science Centers. Special thanks to my local ambassadors Zhoe and Emi for making my visit so special 🥰🥰
An honor and pleasure to be at the @HKLaureatesForum, a “meeting of inspirational minds” gathering @ShawPrize recipients 🏅 and young scientists. Inspirational to hear other laureates and interact with promising researchers curious about the world around them, big 🪐 & small 🧬
November 14, 2025 at 3:24 AM
An honor and pleasure to be at the @HKLaureatesForum, a “meeting of inspirational minds” gathering @ShawPrize recipients 🏅 and young scientists. Inspirational to hear other laureates and interact with promising researchers curious about the world around them, big 🪐 & small 🧬
A unique honor to become a Foreign Member of the @royalsociety.org, together with my @ucberkeleyofficial.bsky.social colleague Stuart Russell and old friends like John Briggs, Asia Akhtar, Patrick Cramer or Ruth Lehmann. Learning about great science and science history was a real treat.
July 19, 2025 at 3:54 PM
A unique honor to become a Foreign Member of the @royalsociety.org, together with my @ucberkeleyofficial.bsky.social colleague Stuart Russell and old friends like John Briggs, Asia Akhtar, Patrick Cramer or Ruth Lehmann. Learning about great science and science history was a real treat.
Very grateful to the Menéndez Pelayo International University for awarding me an honorary doctorate last Tuesday, July 8th, in the incomparable setting of the Magdalena Palace (Santander). On Monday I enjoyed a conversation with those interested in my career and science.
July 19, 2025 at 3:50 PM
Very grateful to the Menéndez Pelayo International University for awarding me an honorary doctorate last Tuesday, July 8th, in the incomparable setting of the Magdalena Palace (Santander). On Monday I enjoyed a conversation with those interested in my career and science.
Yesterday I had the pleasure to visit the Institute for Material Science in Madrid @icmm-csic.bsky.social. Thank you to Dr. Pedro Serana for his invitation to talk about cryo-EM and our latest microtubule studies 🧩🔬🧬
June 24, 2025 at 8:51 PM
Yesterday I had the pleasure to visit the Institute for Material Science in Madrid @icmm-csic.bsky.social. Thank you to Dr. Pedro Serana for his invitation to talk about cryo-EM and our latest microtubule studies 🧩🔬🧬
I spent a wonderful day in the Universidad de Córdoba at an interactive breakfast with faculty and students and giving a science presentation. Lovely to meet its Chancellor, Manuel Torralbo, and my friend and past collaborator Prof. Tina Domínguez-Martín.
June 24, 2025 at 8:48 PM
I spent a wonderful day in the Universidad de Córdoba at an interactive breakfast with faculty and students and giving a science presentation. Lovely to meet its Chancellor, Manuel Torralbo, and my friend and past collaborator Prof. Tina Domínguez-Martín.
Excited to have participated in the Third Symposium of Physical Chemistry in the Universidad Autónoma de Madrid @uam.es last week and honored that the Prizes for best student talk and best posters carried my name 🥰☺️
June 24, 2025 at 8:44 PM
Excited to have participated in the Third Symposium of Physical Chemistry in the Universidad Autónoma de Madrid @uam.es last week and honored that the Prizes for best student talk and best posters carried my name 🥰☺️
Que día tan emocionante volver a mi colegio en Colmenar Viejo y recibir el calor de sus profesores y sus alumnos. Gracias por darle mi nombre a vuestra biblioteca! 🥰
June 24, 2025 at 8:43 PM
Que día tan emocionante volver a mi colegio en Colmenar Viejo y recibir el calor de sus profesores y sus alumnos. Gracias por darle mi nombre a vuestra biblioteca! 🥰
Deeply touched and honored by the Universidad Autónoma de Madrid, my alma mater, (@uam.es) making me Doctor Honoris Causa. Thank you to the Department of Physical Chemistry for nominating me for this recognition. It was a memorable day for me and my family👩🎓
June 24, 2025 at 7:53 PM
Deeply touched and honored by the Universidad Autónoma de Madrid, my alma mater, (@uam.es) making me Doctor Honoris Causa. Thank you to the Department of Physical Chemistry for nominating me for this recognition. It was a memorable day for me and my family👩🎓
Like a dream, my name next to that of @isabelallende.com, and to be recognized by Universidad Internacional Menéndez Pelayo UIMP. Soon after, Irene Vallejo @irenevalmore.bsky.social will receive the same honor. I feel proud to be linked to these truly inspiring, talented women! Thank you UIMP!
June 24, 2025 at 5:03 PM
Like a dream, my name next to that of @isabelallende.com, and to be recognized by Universidad Internacional Menéndez Pelayo UIMP. Soon after, Irene Vallejo @irenevalmore.bsky.social will receive the same honor. I feel proud to be linked to these truly inspiring, talented women! Thank you UIMP!
This week our lab had a double celebration: 1) Eva’s election into the @royalsociety.org and 2) Zhenlin’s new job in Southern University of Science and Technology, China. He will be missed but we wish him success and joy in his independent career!
May 23, 2025 at 1:17 AM
This week our lab had a double celebration: 1) Eva’s election into the @royalsociety.org and 2) Zhenlin’s new job in Southern University of Science and Technology, China. He will be missed but we wish him success and joy in his independent career!
Beyond a role in slowing down Pol II elongation, RECQL5 is known to decrease transcription stress. We propose that RECQL5 may help restart transcription from stalled Pol II by allosterically modulating Pol II’s translocation state. 6/7
January 31, 2025 at 3:24 AM
Beyond a role in slowing down Pol II elongation, RECQL5 is known to decrease transcription stress. We propose that RECQL5 may help restart transcription from stalled Pol II by allosterically modulating Pol II’s translocation state. 6/7
Nucleotide binding (AMPPNP or ADP) causes the RECQL5 helicase domain to undergo a conformational change, pulling on the DNA to induce Pol II to shift to a post-translocation state. Meanwhile, the IRI module seems to serve as a stable anchor for RECQL5 binding. 5/7
January 31, 2025 at 3:24 AM
Nucleotide binding (AMPPNP or ADP) causes the RECQL5 helicase domain to undergo a conformational change, pulling on the DNA to induce Pol II to shift to a post-translocation state. Meanwhile, the IRI module seems to serve as a stable anchor for RECQL5 binding. 5/7
The high resolution of our structures also enabled us to ask how RECQL5 affects Pol II, in particular its translocation state on a DNA/RNA scaffold. We found that Pol II occupies different translocation states when RECQL5 helicase is in its apo vs. nucleotide-bound form. 4/7
January 31, 2025 at 3:24 AM
The high resolution of our structures also enabled us to ask how RECQL5 affects Pol II, in particular its translocation state on a DNA/RNA scaffold. We found that Pol II occupies different translocation states when RECQL5 helicase is in its apo vs. nucleotide-bound form. 4/7
We report high-resolution structures of Pol II elongation complexes with RECQL5, visualizing interactions between RECQL5, Pol II, and DNA, confirming its role as a transcriptional roadblock. This required extensive data processing, as RECQL5 is very flexible! 3/7
January 31, 2025 at 3:24 AM
We report high-resolution structures of Pol II elongation complexes with RECQL5, visualizing interactions between RECQL5, Pol II, and DNA, confirming its role as a transcriptional roadblock. This required extensive data processing, as RECQL5 is very flexible! 3/7
Our microtubule team at lunch with @ruizhangmt.bsky.social, who gave a spectacular seminar at UC Berkeley on his work on axonemes
January 12, 2025 at 6:40 PM
Our microtubule team at lunch with @ruizhangmt.bsky.social, who gave a spectacular seminar at UC Berkeley on his work on axonemes
The Cryo-EM: the next 50 years Symposium held yesterday at @ucberkeleyofficial.bsky.social was a smashing success!!
January 12, 2025 at 4:51 PM
The Cryo-EM: the next 50 years Symposium held yesterday at @ucberkeleyofficial.bsky.social was a smashing success!!
These two distinct inhibition mechanisms are determined by the unique location of H3K4me3 and H3K36me3 along the histone H3 tail and target two essential requirements for efficient trimethylation of histone H3K27 to exclude PRC2 activity from actively transcribe genes
January 8, 2025 at 1:46 AM
These two distinct inhibition mechanisms are determined by the unique location of H3K4me3 and H3K36me3 along the histone H3 tail and target two essential requirements for efficient trimethylation of histone H3K27 to exclude PRC2 activity from actively transcribe genes
The H3K4me3 modification binds to the well-characterized allosteric site in EED and can compete with allosteric activators of PRC2, however it lacks interactions with the EZH2 stimulatory response motif (SRM) and does not trigger allosteric activation like known PRC2 activators.
January 8, 2025 at 1:45 AM
The H3K4me3 modification binds to the well-characterized allosteric site in EED and can compete with allosteric activators of PRC2, however it lacks interactions with the EZH2 stimulatory response motif (SRM) and does not trigger allosteric activation like known PRC2 activators.
The tail-disengaged state reveals a novel binding geometry between PRC2 and the nucleosome that is incompatible with catalytic activity
January 8, 2025 at 1:41 AM
The tail-disengaged state reveals a novel binding geometry between PRC2 and the nucleosome that is incompatible with catalytic activity
We identified two states in cryo-EM analysis of PRC2 bound to H3K36me3 nucleosomes: 1) tail-engaged state that shows accommodation of modified H3 tails in the active site and 2) a tail-disengaged state that indicates H3K36me3 tails engage less efficiently with the PRC2 active site
January 8, 2025 at 1:41 AM
We identified two states in cryo-EM analysis of PRC2 bound to H3K36me3 nucleosomes: 1) tail-engaged state that shows accommodation of modified H3 tails in the active site and 2) a tail-disengaged state that indicates H3K36me3 tails engage less efficiently with the PRC2 active site
We are excited to announce the first Salamanca Biophysics Summer Course organized by Carlos Bustamante and Eva Nogales. See the flyer below and consider applying!
November 26, 2024 at 7:57 PM
We are excited to announce the first Salamanca Biophysics Summer Course organized by Carlos Bustamante and Eva Nogales. See the flyer below and consider applying!