Thank you so much Sandra 🙏

Thanks dear and thank you for all the help and advice 😘

Thanks Rod 🙏

Thanks so much Jen!!

10/n And like any good paper, this is just the beginning for many cool followup projects where we delve into the mechanism and functional exploration of candidate enhancers. Cool stuff in the pipe, stay tuned!!!
We want to thank the reviewers for the constructive advice 🙏

9/n Last but not least, we performed ATAC TFs footprinting to look for TF occupancy on the regulatory regions. While we found the usual suspects in Sertoli cells: SOX, DMRT, NR5A1, the top occupied motif in granulosa cells was that of Lhx9/Emx2!!

8/n In addition, we conducted computational linkage analysis linking genes to their regulatory elements in a positive (enhancer) or negative manner (silencer).

7/n We then applied Promoter Capture HiC on E13.5 Sertoli and granulosa cells and were able to identify physical proximity between enhancers and their target genes. This was done with the superb Lera Malysheva.

6/n We then focused on regulatory elements. We identified numerous regulatory elements that are specific to the sex (testis/ ovaries) or to the developmental time

5/n We employed ATAC-seq and RNA-seq on these cells to identify their gene expression and functional regulatory elements. We provide a cool tool that enables you to track the expression of your gene of interest. Check this out:
4ljrl2-roni-weiss.shinyapps.io/tpm_allgenes/

4/n To start looking at that we started a project 5 years ago that came into completion now. We used existing and new transgenic mouse lines that enable u to purify Sertoli and granulosa cells during 4 key time pints in which the sex is being determined (E11.5-15.5)

3/n So, I believe that a lot of the action is mediated via the non-coding parts of the genome, but where exactly??? it is 98% of the genome!

2/n So you all know that I am obsessed about my precious Enh13, deletions/duplications of which lead to XY and XX sex reversal in mice and human. And, we know that most DSD patients fail to receive genetic diagnosis even after whole exome sequencing.

Congrats Vicki, super interesting!