We searched for trends at specific sites and found examples of how a plasmid can be stable and maintain its structure for months, and also how a single event (recombination with a co-resident plasmid) can dramatically change plasmid structure and consequently classification by MOB-suite/pling

7/8

We found 11.4 % of isolates encoded both NDM and OXA-48 in the same cell. Nine plasmids encoded both NDM and OXA-48-type genes on the same backbone, which haven't been seen much in the lit yet. Five were in Kleb pneumo ST16 on an IncX3 backbone

6/8

Most of the NDM plasmids in our dataset were IncF-type (not a popular type in other studies) and had multiple #AMR genes and mobile elements. This made it impossible to predict plasmid clusters for isolates with short-read only data

5/8

We had a big dataset (1032 isolates, 226 complete NDM plasmids) over 14 years of #AMR surveillance data from the Canadian Nosocomial Infection Surveillance Program (CNISP)

2/8

Enjoying #APBHM25 - lots of super talks and excited to try out all the new tools!

I'm virtually presenting poster # 115 on our lab & bioinf workflow to rapidly identify bacteria & fungi organisms and AMR determinants from positive blood cultures, check it out here: github.com/lerminin/abp...
1/3