4 and 1 color systems are all being discontinued…

I believe all 1 and 4 color systems are now EOL, so it’s all be 2 color soon.

The problem is the MinION doesn’t seem to produce significant revenue and MinION revenue is going down not up. It would be unfortunate for users, but the correct strategy might be to cancel MinION/flongle and push more users to PromethION.

You’ve repeatedly asked to leave the ASeq Discord (where this comment was posted) in part because you quote without citing (like this), even when users have specifically request it.

Please leave the Discord. Sticking around in a forum you’ve been asked to leave is fairly creepy behavior.

One limitation of the MiSeq is that it only has a y stage not a full xy stage. Would either be limiting or requiring retrofitting I guess. But will be fun finding new applications for MiSeqs as they come on the market due to discontinuation!

I bought one of these up a while back. If there’s anything I can help with give me a shout (new@sgenomics.org).

They showed 800bp read length average on Simplex and 350bp on duplex in the Webinar (which is full runs, but maybe cherry picked).

The preprint is 4 years out of date.

They showed single reads in the 2Kb range. One run with a simplex 800bp average IIRC. My guess is they can probably get a reasonable population at 2Kb currently but we don’t yet have good data from Roche on where the read length tops out.

The preprint is 2020 data. The current data they presented in the webinar was >99% accuracy. IIRC 99.3% raw. Duplex lets you filter a lot of those errors getting you to Q39 on concordant bases they say.

Confused, it’s already been installed and used at the Broad and another institute. Shows ~Q20 average on simplex Q39 on concordant duplex bases.

Can’t do modifications like ONT, but they’ve suggest they may be able to do something (seems hard to me!)

I’ve been writing about eugenics and investors doing Nazi salutes… your day sounds better:

bitsof.bio/p/nucleus-ge...