Mathias Munschauer
@munschauerlab.bsky.social
Professor at Heidelberg University (@uniheidelberg.bsky.social). Director Molecular Virology. Interested in RNA viruses, RNA-binding proteins and RNA medicine. www.munschauerlab.de
Using SHIFTR, we identify interactions of the 5′ and 3′-terminal regions of authentic SARS-CoV-2 RNAs produced during infection and accurately recover known and novel RNA interactors.
February 12, 2024 at 8:09 AM
Using SHIFTR, we identify interactions of the 5′ and 3′-terminal regions of authentic SARS-CoV-2 RNAs produced during infection and accurately recover known and novel RNA interactors.
Proteins bound to a specific RNA can be released from interphase extracts by selectively degrading the RNA of interest, for instance with RNase H + specific DNA probes. Loss of the RNA leads to a SHIFT of crosslinked proteins to the organic phase in the next extraction step.
February 12, 2024 at 8:08 AM
Proteins bound to a specific RNA can be released from interphase extracts by selectively degrading the RNA of interest, for instance with RNase H + specific DNA probes. Loss of the RNA leads to a SHIFT of crosslinked proteins to the organic phase in the next extraction step.