Mark Pownall
@mpownall.bsky.social
https://pownall-lab.org | Sandler Fellow at UCSF | chromatin, transcription, microscopy (ChromExM), development 🐟🧬🔬
Reposted by Mark Pownall
New preprint with @gfudenberg.bsky.social
We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding.
This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇
www.biorxiv.org/content/10.1...
We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding.
This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇
www.biorxiv.org/content/10.1...
NIPBL dosage shapes genome folding by tuning the rate of cohesin loop extrusion
Cohesin loop extrusion is a major driver of chromosome folding, but how its dynamics are controlled to shape the genome remains elusive. Here we disentangle the contributions of the cohesin cofactors ...
www.biorxiv.org
August 16, 2025 at 3:03 AM
New preprint with @gfudenberg.bsky.social
We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding.
This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇
www.biorxiv.org/content/10.1...
We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding.
This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇
www.biorxiv.org/content/10.1...
Reposted by Mark Pownall
Our first expansion microscopy results. Imaged today on "normal" Zeiss LSM 900, revealing chromatin and RNA polymerase II clusters at an unreal level of detail.
Our PhD student @mofrawe.bsky.social learned the protocol from amazing @mpownall.bsky.social 🙏
Months of preparation are paying off now!
Our PhD student @mofrawe.bsky.social learned the protocol from amazing @mpownall.bsky.social 🙏
Months of preparation are paying off now!
August 14, 2025 at 6:28 PM
Our first expansion microscopy results. Imaged today on "normal" Zeiss LSM 900, revealing chromatin and RNA polymerase II clusters at an unreal level of detail.
Our PhD student @mofrawe.bsky.social learned the protocol from amazing @mpownall.bsky.social 🙏
Months of preparation are paying off now!
Our PhD student @mofrawe.bsky.social learned the protocol from amazing @mpownall.bsky.social 🙏
Months of preparation are paying off now!
Reposted by Mark Pownall
Another paper bluetorial! Today: how does the spatial location of genes influence their function? (1/n) www.nature.com/articles/s41...
The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate - Nature Cell Biology
Marin et al. report the role of lamin proteins and the lamin B receptor (LBR) in chromatin positioning at the nuclear periphery. Knockout of all lamins and LBR in mouse embryonic stem cells leads to h...
www.nature.com
July 22, 2025 at 5:10 PM
Another paper bluetorial! Today: how does the spatial location of genes influence their function? (1/n) www.nature.com/articles/s41...
Reposted by Mark Pownall
Excited to share our new paper in @cp-molcell.bsky.social Molecular Cell: "Catalytic-dependent and independent functions of the histone acetyltransferase CBP promote pioneer-factor-mediated zygotic genome activation" — the big part of my PhD! www.cell.com/molecular-ce...
Catalytic-dependent and independent functions of the histone acetyltransferase CBP promote pioneer-factor-mediated zygotic genome activation
Marsh, Pirogov, et al. use the early Drosophila embryo to demonstrate that the conserved
histone acetyltransferase CBP is required for pioneer-factor-mediated transcriptional
activation of the zygotic...
www.cell.com
May 30, 2025 at 12:52 PM
Excited to share our new paper in @cp-molcell.bsky.social Molecular Cell: "Catalytic-dependent and independent functions of the histone acetyltransferase CBP promote pioneer-factor-mediated zygotic genome activation" — the big part of my PhD! www.cell.com/molecular-ce...
First paper from the Pownall lab calls for a first post on bluesky! Check out our mini review on nanoscale imaging of the nucleus in vivo. We are excited to address some of these open questions and technical challenges through future work from the lab—stay tuned!
portlandpress.com/biochemsoctr...
portlandpress.com/biochemsoctr...
Microscopy methods for the in vivo study of nanoscale nuclear organization
Eukaryotic genomes are highly compacted within the nucleus and organized into complex 3D structures across various genomic and physical scales. Organization within the nucleus plays a key role in gene...
portlandpress.com
February 4, 2025 at 12:25 AM
First paper from the Pownall lab calls for a first post on bluesky! Check out our mini review on nanoscale imaging of the nucleus in vivo. We are excited to address some of these open questions and technical challenges through future work from the lab—stay tuned!
portlandpress.com/biochemsoctr...
portlandpress.com/biochemsoctr...