Mathias Mahn
@mmahn.bsky.social
Trying to bridge cellular, circuit, and behavior neuroscience - from designing molecular tools to studying complex behaviors.
Please share: the FMI is hiring a tenure-track Group Leader in Multicellular Systems to explore the molecular and cellular mechanisms driving the formation, maintenance, or destabilization of tissues, organs and organisms. Apply at: www.fmi.ch/education-ca...
December 17, 2024 at 6:53 AM
Please share: the FMI is hiring a tenure-track Group Leader in Multicellular Systems to explore the molecular and cellular mechanisms driving the formation, maintenance, or destabilization of tissues, organs and organisms. Apply at: www.fmi.ch/education-ca...
Reposted by Mathias Mahn
How to rebuild your network on @bsky.app and improve your experience here?
I made a short starter guide with resources.
www.neuronaldynamics.eu/resources/bl...
Also, a thread 🧵 below.
I made a short starter guide with resources.
www.neuronaldynamics.eu/resources/bl...
Also, a thread 🧵 below.
November 17, 2024 at 2:01 PM
How to rebuild your network on @bsky.app and improve your experience here?
I made a short starter guide with resources.
www.neuronaldynamics.eu/resources/bl...
Also, a thread 🧵 below.
I made a short starter guide with resources.
www.neuronaldynamics.eu/resources/bl...
Also, a thread 🧵 below.
Reposted by Mathias Mahn
Many people have asked me what's the deal with "SIO" in our Addgene vectors (www.addgene.org/Ofer_Yizhar/) - we use these in most of our Cre-dependent AAVs - so here's a brief explanation of how it works.
www.addgene.org
November 28, 2024 at 6:12 PM
Many people have asked me what's the deal with "SIO" in our Addgene vectors (www.addgene.org/Ofer_Yizhar/) - we use these in most of our Cre-dependent AAVs - so here's a brief explanation of how it works.
One difference is that the DIO approach leads to the permanent removal of DNA, making it irreversible. For SIO an equilibrium between forward and reverse recombinantion is created. However the reverse probability is low, creating a bias to the activated state. In practice expression levels are good.
The lox66/71 idea is not new - it came from this paper: pmc.ncbi.nlm.nih.gov/articles/PMC... by Beat Lutz. It was just never used in AAV as far as we know. The idea is that after Cre recombination between these two sites, the resulting sites are no longer recognized by Cre and can't recombine again
November 29, 2024 at 8:50 AM
One difference is that the DIO approach leads to the permanent removal of DNA, making it irreversible. For SIO an equilibrium between forward and reverse recombinantion is created. However the reverse probability is low, creating a bias to the activated state. In practice expression levels are good.
Do you want to be my "new sciencetwitter" friend? 🤗
November 13, 2024 at 9:40 PM
Do you want to be my "new sciencetwitter" friend? 🤗