Shutt Lab
@mitomorph.bsky.social
Associate Professor at University of Calgary studying mitochondria.
Congrats to ShuttLab PhD student Mashiat Zaman @mashiatzaman.bsky.social on receiving an award for his short talk at the recent CSMB-PRinCE meeting in Ottawa
June 3, 2025 at 9:54 PM
Congrats to ShuttLab PhD student Mashiat Zaman @mashiatzaman.bsky.social on receiving an award for his short talk at the recent CSMB-PRinCE meeting in Ottawa
Finally, through collaborations, we show that the reporter works in budding yeast (with Kurt Schmoller's lab) and in Drosophila (Mashiat again, with help from Abhi Sharma in Savraj Grewal's lab).
*However, there are caveats associated with high expression, which can be detrimental.
January 24, 2025 at 5:47 PM
Finally, through collaborations, we show that the reporter works in budding yeast (with Kurt Schmoller's lab) and in Drosophila (Mashiat again, with help from Abhi Sharma in Savraj Grewal's lab).
*However, there are caveats associated with high expression, which can be detrimental.
Mashiat also showed we can image extramitochondrial mtDNA and follow mtDNA in extracellular vesicles.
January 24, 2025 at 5:47 PM
Mashiat also showed we can image extramitochondrial mtDNA and follow mtDNA in extracellular vesicles.
In the updated version, we show that we can use the reporter to quantify changes in mtDNA size and number (Mashiat Zaman in the Shutt lab).
#FluorescenceFriday
#FluorescenceFriday
January 24, 2025 at 5:47 PM
In the updated version, we show that we can use the reporter to quantify changes in mtDNA size and number (Mashiat Zaman in the Shutt lab).
#FluorescenceFriday
#FluorescenceFriday
Given the known links between mtDNA and inflammation, and the fact that inflammation can cause myopathy, we followed up in more detail, and found evidence for activation of both TLR9 and cGAS-STING inflammatory pathways.
June 27, 2024 at 8:01 PM
Given the known links between mtDNA and inflammation, and the fact that inflammation can cause myopathy, we followed up in more detail, and found evidence for activation of both TLR9 and cGAS-STING inflammatory pathways.
When we characterized patient fibroblasts, we observed several phenotypes associated with MFN2 dysfunction. Excitingly, we also observed mtDNA puncta that were outside of the mitochondrial network and which co-localized with early endosomes.
June 27, 2024 at 8:01 PM
When we characterized patient fibroblasts, we observed several phenotypes associated with MFN2 dysfunction. Excitingly, we also observed mtDNA puncta that were outside of the mitochondrial network and which co-localized with early endosomes.
For anyone attending ASCB who wants to learn more about our new mtDNA probe - Co-author and Collaborator Pina Colarusso will be presenting a poster Tuesday afternoon:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
December 2, 2023 at 12:02 AM
For anyone attending ASCB who wants to learn more about our new mtDNA probe - Co-author and Collaborator Pina Colarusso will be presenting a poster Tuesday afternoon:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
What was really great, was that we were able to photoactivate a subset of mtDNA nucleoids (magenta), which we could follow over time.
October 30, 2023 at 10:19 PM
What was really great, was that we were able to photoactivate a subset of mtDNA nucleoids (magenta), which we could follow over time.
Next, we tried a different fluorescent protein, Kaede, which is photoswitchable from green to red. To our surprise, the mt-Kaede-HI-NESS worked beautifully, even with just a single H-NS DNA binding domain. (Mitos in grey and Kaede in cyan)
October 30, 2023 at 10:17 PM
Next, we tried a different fluorescent protein, Kaede, which is photoswitchable from green to red. To our surprise, the mt-Kaede-HI-NESS worked beautifully, even with just a single H-NS DNA binding domain. (Mitos in grey and Kaede in cyan)
Excitingly, we were able to reduce the background mitochondrial network signal by adding a second H-NS DNA binding domain to get better nucleoid signal. Here you can see colocalization of TagRFP (magenta) with SYBR Gold labelled mtDNA nucleoids (cyan)
October 30, 2023 at 10:16 PM
Excitingly, we were able to reduce the background mitochondrial network signal by adding a second H-NS DNA binding domain to get better nucleoid signal. Here you can see colocalization of TagRFP (magenta) with SYBR Gold labelled mtDNA nucleoids (cyan)
Our initial construct, mt-TagRFP-HI-NESS, worked OK. We could see nucleoid signal - Here you can see the TagRFP signal in puncta (magenta) within the mt network (grey) labelled with Mitotracker green. But there was also some background mitochondrial network signal in some cells.
October 30, 2023 at 10:13 PM
Our initial construct, mt-TagRFP-HI-NESS, worked OK. We could see nucleoid signal - Here you can see the TagRFP signal in puncta (magenta) within the mt network (grey) labelled with Mitotracker green. But there was also some background mitochondrial network signal in some cells.