Beautiful, congratulations! to you and all the Dutch cryo-EM and scientific community!

Related reading:
1. A recent preprint by Cristing Gutterrez-Vargas et al with several structures of rodent ribosomes, including the naked mole rat, biorxiv.org/content/10.1...
2. The classical 2013 paper with functional characterization of the NMR ribosome: pmc.ncbi.nlm.nih.gov/articles/PMC...

No spoilers this time, just the beautiful structure!

Mehmet Gul led the work with the dream team of Alice Rossi, Christian Spahn, and Gary Lewin.

Enjoy it and have a fantastic day

It's a team effort of the group: Igor, Ulja, Xiaofeng, Artsemi, Patricia, and we thank the funders.

The print version will come up in a book on methods in molecular biology, hopefully later this year!

Enjoy reading and have a great week!

But for a larger system, smaller proteins, interactions with the cytoskeleton, cluster formation, and other biological applications, there seems to be a use of this microvesicle technique.

Some thoughts: wonderful papers by Roderick Mackinon and other groups show that 2D imaging of membrane proteins in small vesicles can result in high-resolution structures. So if you want structures - try that!

pubmed.ncbi.nlm.nih.gov/37098056/

The book chapter is a set of methods to express your membrane protein of interest, purify vesicles, image them by cryo-ET, and perform subtomogram averaging.

As usual, we like to go into details:

The chapter is inspired by our recent paper, where we show that tetrameric and pentameric forms of the serotonin receptor ion channel 5-HT3R were present in the same membrane patches
www.embopress.org/doi/full/10....

Roses are red
Because they have SCREP!

Thanks Mimi! ❄️❄️❄️

Thanks Sandip!

Thanks John !!!

I think that it is not compatible with Krios 5

yeah, its very sad that Thermo makes older devices non-compatible.

Thanks!