Full paper (to appear in NeurIPS 2025!) here: arxiv.org/abs/2506.00138

Made possible only by the unique environment and amazing community at Janelia and the Gatsby. @hhmijanelia.bsky.social @gatsbyucl.bsky.social

Feedback, ideas, or requests for transgenic lines, protocols, and code are warmly welcome!

Finally, Virginia @vmsruetten.bsky.social is now on the job market, ready to expand WHOLISTIC into a larger research program. Any institute will be lucky to hire her.

Thanks to all authors, including Wei Zheng and Guoqiang Yu, Amy Hu, and the Janelia @hhmijanelia.bsky.social and 4DCP communities, Maneesh Sahani, and others.

WHOLISTIC bridges cellular biology with whole organism physiology and behavior, and we believe will open up new avenues for studying vertebrate brain-body coordination, discovering new modes of long-range communication, and more.

Check out the website for more details: wholistic.janelia.org

and with Igor Siwanowicz are working towards whole-body atlases.

Together with Paul Tillberg, we advanced Whole Body Expansion Microscopy to access the subcellular scales of the organism and match cellular dynamics to their molecular substrate,

Combining WHOLISTIC with optogenetics, correlative brain–body dynamics can be tested causally, yielding a topological mapping between brain and body.

and coupled activation of motor circuits and muscle groups, as well as responses in other tissues, including the skin.