Thanks to all authors, including Wei Zheng and Guoqiang Yu, Amy Hu, and the Janelia @hhmijanelia.bsky.social and 4DCP communities, Maneesh Sahani, and others.

and with Igor Siwanowicz are working towards whole-body atlases.

Together with Paul Tillberg, we advanced Whole Body Expansion Microscopy to access the subcellular scales of the organism and match cellular dynamics to their molecular substrate,

visualizes gut peristalsis together with enteric nervous system activity,

skin epithelium (video 100x sped up),

WHOLISTIC reveals many phenomena, including traveling waves along the kidney,

Preprint -
Excited to present WHOLISTIC, which extends the concept of whole-brain functional imaging to the entire body. Pioneering work by incredibly talented Virginia Ruetten @vmsruetten.bsky.social, this platform reveals whole-organism cellular dynamics in vivo.
www.biorxiv.org/content/10.1...

Background from past work: We knew that during normal giving-up behavior, NE activates astrocytes as fish struggle to regain control. After about 20 seconds of this failure integration, astrocytes then activate neurons that shut off swim attempts. Ketamine's effects looked a bit similar. Old movie:

Imaging neurons and astroglia during ketamine exposure showed strong activation of noradrenergic (NE) neurons in the hindbrain. Astrocytes were activated through NE receptors, and for about 20 minutes, were in a hyper-excited state: