Thanks Martina! :-)

5) Thanks again to all authors, especially Mick lab grad student Tommy Sroka and PostDoc Avishek Prasai, as well as valetrivi.bsky.social and Josefine Hoeren from the Feistel lab who established iAPEX in developing Xenopus brains.

4) iAPEX is more specific than conventional APEX labeling, which is a huge deal for subcellular proteomics of small subdomains. We profiled primary cilia of two different cell types and identify new cilia candidate proteins. Our data confirms heterogeneity in cilia proteomes of different cell types.

3) Our collaborator @biancaschrul.bsky.social demonstrated this by selectively activating a UBXD8-population that localizes on the surface of #LipidDroplets. We think this approach has lots of potential to investigate organellar contact sites.

2) We can use the iAPEX enzymatic cascade to activate subpopulations of APEX enzymes in subcellular compartments, specifically where they co-localize with DAAO. We can show this for #mitochondria, subdomains of cilia (cilia tips), as well as for proteins that localize to two different compartments.

1) By using a D-amino acid oxidase (DAAO), iAPEX omits the requirement of external H2O2 addition and therefore reduces toxicity and allows in vivo applications, which our fantastic collaborators @kerstinfeistel.bsky.social and @walenteklab.bsky.social could show in developing #Xenopus embryos.

Congrats all, in particular Rudi! Love the paper.

Everybody in my lab is working so hard, lots of things can happen within two days.

Thank you all for a wonderful meeting full of amazing science and inspiring discussions. It was so much fun! Thanks @bastinlab.bsky.social, Sylvie Friant and Séverine Bär for organizing it at such a beautiful location. Can't wait for next time in Lyon!

Deeply saddened to hear about the passing of Jochen. We had very few but absolutely fantastic interactions when he was still in Dresden. He will be missed dearly.

Can I invite myself?