Matyas Bubna-Litic
@matyasbl.bsky.social
Interested in mechanobiology, morphogenesis, organisers and synthetic embryo models.
Research Fellow in Mongera lab UCL | PhD in Mayor and Charras labs | YEN Committee
Research Fellow in Mongera lab UCL | PhD in Mayor and Charras labs | YEN Committee
That's such a great book cover
October 4, 2025 at 10:05 PM
That's such a great book cover
Animal caps are back in fashion!
September 22, 2025 at 9:40 PM
Animal caps are back in fashion!
This worked for me:
155 mM NaCl, 10 mM Tris-HCl at pH 7.5, 50 mM NaBH4 > in refrigerator 2 days before use.
Use: After permeabilising apply AF-reducing agent overnight at 4˚C
It's possible to do it faster at room temp and higher NaBH4 conc but it's less gentle to the sample.
Hope it works!
155 mM NaCl, 10 mM Tris-HCl at pH 7.5, 50 mM NaBH4 > in refrigerator 2 days before use.
Use: After permeabilising apply AF-reducing agent overnight at 4˚C
It's possible to do it faster at room temp and higher NaBH4 conc but it's less gentle to the sample.
Hope it works!
August 10, 2025 at 10:51 PM
This worked for me:
155 mM NaCl, 10 mM Tris-HCl at pH 7.5, 50 mM NaBH4 > in refrigerator 2 days before use.
Use: After permeabilising apply AF-reducing agent overnight at 4˚C
It's possible to do it faster at room temp and higher NaBH4 conc but it's less gentle to the sample.
Hope it works!
155 mM NaCl, 10 mM Tris-HCl at pH 7.5, 50 mM NaBH4 > in refrigerator 2 days before use.
Use: After permeabilising apply AF-reducing agent overnight at 4˚C
It's possible to do it faster at room temp and higher NaBH4 conc but it's less gentle to the sample.
Hope it works!
Looks like an exciting meeting. Would you know if it's possible to attend online? Thanks
August 10, 2025 at 9:23 AM
Looks like an exciting meeting. Would you know if it's possible to attend online? Thanks
I'm not sure how bacteria on oysters are but in early Xenopus cells sodium borohydride works well to remove autofluorescence from sources like PFA fixation. There's a good protocol on CSH I can try look for it.
August 9, 2025 at 10:50 PM
I'm not sure how bacteria on oysters are but in early Xenopus cells sodium borohydride works well to remove autofluorescence from sources like PFA fixation. There's a good protocol on CSH I can try look for it.
Based on 'Tracing the genetic footprints of vertebrate landing in non-teleost ray-finned fishes' by Bi et al 2021
August 6, 2025 at 12:38 PM
Based on 'Tracing the genetic footprints of vertebrate landing in non-teleost ray-finned fishes' by Bi et al 2021
It therefore makes sense to me that what we generally call ‘fish’ should be termed ‘non-tetrapod bony fish’ which excludes land-dwelling four-legged animals like mammals (including whales) and reptiles as well as sharks.
August 6, 2025 at 11:12 AM
It therefore makes sense to me that what we generally call ‘fish’ should be termed ‘non-tetrapod bony fish’ which excludes land-dwelling four-legged animals like mammals (including whales) and reptiles as well as sharks.
People have started to accept the scientific view that birds have descended from dinosaurs and there is no way to group dinosaurs without including the bird lineage. Therefore, we now often refer to the extinct species of lizard-like animals who dominated the Mesozoic Era as ‘non-avian dinosaurs’.
August 6, 2025 at 11:12 AM
People have started to accept the scientific view that birds have descended from dinosaurs and there is no way to group dinosaurs without including the bird lineage. Therefore, we now often refer to the extinct species of lizard-like animals who dominated the Mesozoic Era as ‘non-avian dinosaurs’.