🧬 🖥️ #bioinformatics #python #R

I did a lot of validation work to show that the approach works when using pseudobulked data. I even implemented bootstrapping to get estimates of confidence. This all got bundled into an R package which I tried to make pretty easy to use. This is an osteo patient sample.

This was in collaboration with John Sherman and Nixon Raj (not on bluesky) who are covering most of the material.

One reason I tend to use the same seed everywhere is I don't want to give the appearance of fishing for specific results. If I do this in R:
set.seed(784387803)
t.test(sample(1:100, 10), sample(1:100, 10))
I get a super significant result because I wrote a loop to find a good seed.

Your lab logo is fantastic

How do you 'make noise' online about your professional work? Let me know and I’ll share it with the folks I talked to last week so they hear from people who are better at this than me!

Here’s the result of my own effort (putting my money where my mouth is!):
My GitHub profile: github.com/MVesuviusC
My new personal site: mvesuviusc.github.io

I'm naturally bad at this, but getting better - if I can do it, anyone can!
A key tool I use for this is GitHub, which makes it much easier.
I walked through setting up a GitHub profile page to showcase your work. I also showed how GitHub Pages lets you create a simple personal website, quickly.

You need to make a bit of noise to show people you exist and what you can do.
One advantage for computational scientists is how easily we can show off our work online. When I did lab work, it would have been hard to show myself pipetting PCRs on the internet.

I'll also add that I've seen fastqs in SRA for scRNAseq datasets that lack the data on barcodes and is functionally just expensive bulk RNAseq

@franckdumetz.bsky.social

better link to the text: github.com/jwiegley/git...

One thing I found helpful to understand git more in depth was a series of podcast episodes going over jwiegley.github.io/git-from-the.... First episode is here: www.codingblocks.net/podcast/unde...

I can confirm that Genevieve is awesome to work with