Nice talk by @dralhubb.bsky.social at #microbio25. Urobiome as a possible reservoir of AMR for UTIs, including HGT and selection of spontaneous mutants!

Looking forward to speaking in the AMR mechanisms and regulation session at #Microbio25 today. I'll be speaking right after @brockhurstlab.bsky.social who will discuss our within-patient resistance evolution project, and I'll outline some measures we can take against resistance evolution in-host

Absolute treat to see @relenski.bsky.social tell the story of 37 years of science and evolution at #microbio25, a proper 'conference bucket list' moment for me, reading the LTEE story as an undergrad definitely motivated me to get into experimental evolution!

Great talk on the rising issue of biocide tolerance by Vicky Bennet in the AMR mechanisms and regulation session - multiple mutations from evolution experiments of Proteus mirabilis match those seen in clinical isolates!

Great talk by @mermanchester.bsky.social member @whelanfj.bsky.social at Microbiology Society annual conference - insights on 600 cumulative years of P. aeruginosa within-patient evolution by the prairie epidemic strain! 🤯

Great to visit Nottingham Trent University today to give a seminar on our within-patient resistance evolution work. Thanks to @dralhubb.bsky.social for the invite and all the interest in our work!

Jet lag has mostly worn off now - very pleased to be in the beautiful Puerto Vallarta for #SMBE24. Excited to be speaking in the one-health and microbial evolution session tommorrow, on the within-patient evolution of antimicrobial resistance!

Nice articles in the Guardian today on the AMR crisis, though this explainer of how AMR happens was a little painful to read - Anyone in sci-comm know why the 'process' often isn't named - evolution? Can see the idea of getting the point across clearly re: drug over-use though. #MicroSky

Many trials to not document AMR as a response, despite its increasing clinical importance. Routine biobanking of samples taken prior to and during a trial, would allow for follow up investigations of within-patient AMR response to the novel antimicrobial therapy. 6/7

We finish by making the case that clinical trials of antimicrobials hold underutilised potential for understanding within-patient emergence of AMR - offering an ideal setting for developing a quantitative understanding of how resistance emerges during therapy 5/7

We highlight however that current understanding is somewhat limited by a lack of scale in past work - evidence is piecemeal, dominated by single-patient case studies, and often does not feature sampling sufficient to conclusively rule out or rule in evolutionary mechanisms 4/7

We also discuss how the prevailing mechanism likely varies between infecting organism, infection site within the body, infection duration and bacterial lifestyle 3/7

We summarise evidence for four prevailing mechanisms of AMR emergence within-patient: i) Spontaneous mutation, ii) Horizontal transfer (de novo mechanisms), iii) Selection of existing resistance, and iv) immigration of resistant lineages from elsewhere (ecological mechanisms) 2/7

The main paper from my PhD is now available in ceramic form! Nice surprise gift from @taylorlabgroup.bsky.social - a lot of coffee was consumed whilst writing these words, and now they will hold coffee to support future writing!

Excited to be at @meehubs.bsky.social 2024! I'll be making a last minute entry to the speaker line-up tomorrow at the Oxford Hub, at 11am UK time. Also check out @brockhurstlab.bsky.social PhD student @taoranfu.bsky.social awesome poster (311), on effects of oxidative stress on QS in P. aeruginosa!

On my way to Birmingham for BSAC antimicrobial resistance mechanisms workshop! Excited to be making a late entry to the speaker lineup > I'll be discussing our research on within-patient evolution of ciprofloxacin resistance during a clinical trial, tomorrow morning! #microsky