Proud to see your story featured on the cover of #Neuron! 🤩

Along with a great #preview from Fides Zenk lab @epign.bsky.social

www.cell.com/neuron/abstr...

Intriguingly, pharmacological PRC2 inhibition in human cortical organoids induces increased production of the ECM components Syndecan 1 and Laminin alpha 1. (9/11)

Our cell type-specific profiling of the genome-wide H3K27me3 profile not only identified neuronal differentiation, but also extra-cellular matrix (ECM) genes as targets of PRC2. (7/11)

We found that loss of H3K27me3 in human cortical organoids induces a shift of neural progenitor cell proliferation towards differentiation that results in reduced organoid size. (5/11)

Here, we performed pharmacological inhibition of PRC2 in human cortical organoids from week 3 to 8, leading to a near complete loss of H3K27me3, and studied NPC behaviour. (4/11)

We apply an innovative, highly multiplexed approach, Epi-CyTOF, to study > 30 epigenetic modifications at single-cell level in the human developing neocortex. We identify H3K27me3, deposited by PRC2, as the modification with the strongest cell type-specific enrichment. (2/11)

How do epigenetic modifications contribute to human #neocortex #development? Excited to see our work out in #Neuron. We applied Epi-CyTOF and identified a novel role for PRC2 in regulating #ECM.

@crtd-tud.bsky.social @cp-neuron.bsky.social

www.sciencedirect.com/science/arti...

A thread below 👇

Metabolic-epigenetic interplay emerges as a novel direction for studying the timing of cortical development, and brain organoids offer exciting new experimental approaches.

Metabolic and epigenetic regulation are tightly linked. The deposition and removal of epigenetic marks, including DNA and histone modifications, requires substrates provided by a plethora of metabolic pathways.

Cell metabolism differs between cell types and species in cortical development.

Mutations in various histone modifiers cause developmental disorders linked to impaired brain development. These disorders highlight the importance of activating and repressive histone modifications in controlling human cortical development.

The neocortexes of mice and humans differ in various respects, including developmental timing. Epigenetic regulation has emerged as an important mechanism to control developmental timing.

💫 New preprint from the lab: Comparing great ape cerebral organoids, we found that human-specific morphoregulatory signatures in basal radial glia characterise neocortex evolution. Fantastic work from super-talented PhD student Theresa Schütze!

www.biorxiv.org/content/10.1...

How do different #PRC1 complexes contribute to the regulation of #NSC fate? Side-by-side comparison of canonical and non-canonical PRC1 in #proliferation and #differentiation.

Our study is now published:
www.life-science-alliance.org/content/8/4/...

By Janine Hoffmann @crtd-tud.bsky.social

Planning to attend the German Neuroscience Society Meeting in March 2025 in Göttingen? Consider joining the Satellite Symposium of the Schram Foundation on 25th March 2025!

"THE EVOLUTIONARY TAPESTRY OF COGNITION"
Great lineup of speakers! No registration fee!

www.schram-stiftung.de/symposien

Interested in using sliced cortical organoids for functional studies? Check out our step-by-step protocol for electroporation!

app.jove.com/t/67598/elec...

By our incredibly skilled technician Annika Kolodziejczyk 🤩

💫 Join us! We are looking for an enthusiastic #postdoc! Are you excited about #epigenetics, #neocortex development, neurodevelopmental #disorders, #evolution, #organoids?
Apply until 27 Nov 2024! 4-year fully funded position

tu-dresden.de/vacancy/11767