As always, my unbelievably talented partner
@ShiraHoland
had the punishing job of integrating all of this information into the beautiful figures you see here. Hope you like it! biorxiv.org/content/10.1... 13/13
🧪🍄

This paper has been a long time coming and required a lot of thought and work by everyone in the
@ZivReichLab, especially @rutikapon.bsky.social @reinatnevo
@SRezenman
and Ivgeni Tsigalnitski. 12/13

Stress seldom encountered does not require such a network and its costly upkeep, so strategies such as #bethedging are sufficient and could be refined upon recurring exposures. 11/13

We think then that #creativity in response or stress-induced variability could arise when cells have a robust, redundant network of responses, allowing multiple adaptive avenues to be explored and explored. 10/13

Our hypothetical model of what is going on is quite counterintuitive. NaCl, as it is a very exotic stress, has a minimal proteomic response attributed to it, while pH is an integral part of the yeast way of life. 9/13

A similar picture was conveyed. pH stress, which elicits a high proteomic response, also has high diversity after the onset of stress. In contrast, NaCl stress has minimal variability in proteomic response and maintains the same level of diversity under stress. 8/13

We wanted to probe this phenomenon deeper, so we utilized our #gUMIBEAR barcoding and tracking method to see the population's lineage dynamics at high temporal resolution. 7/13

We saw something quite striking. Not all stresses are the same. For the same morality level, #ShannonEntropy behaved differently, indicating that some stresses, not others, induced the population variability. 6/13

But rather multimodal distributions, exemplifying what we think are multi-state phenotypes. We used #ShannonEntropy to portray these populations accurately and plotted them against the stress in various insult levels. 5/13

Once we had multiple stresses measured on our newly constructed library, we noticed something interesting. Distributions were not what you would expect from noisy populations when stress was administered, 4/13

Stress is not a binomial phenomenon, i.e., it's not only an on-or-off thing but a much more nuanced experience. Testing multiple levels of each perturbation, ranging from mild to lethal, allows us to describe the relationship between the magnitude of stress and variability. 3/13

Many works have described the function of #noise and its relationship to #stress, but we wanted to quantify this relationship in a way that has yet to be widely measured. This required many levels of stressful perturbations. 2/13

Right???

It's about how a cell (yeast), when faced with an unresolved stress, can utilize something similar to reinforcement learning to repurpose old, redundant functions for new stress-alleviating ones. ☺️