Excited to share our work with @markrcookson.bsky.social and Payel Sen labs. We combine #SingleCell and #Nanopore sequencing to profile #RNA isoforms from young to geriatric mouse brains. We uncover #aging changes in transcription, processing, and splicing that also impact coding potential.

#RNAsky

Our paper out in eLife. Full-length direct RNA #nanopore sequencing uncovers 5' #RNA shortening upon cellular #stress that depends on ✅ stress-granules and #XRN1 but ❌ not poly(A) or cap removal elifesciences.org/articles/96284

🤔 useful to further reduce translational load during #ISR?

#RNAbiology

img: Sci-Illustrate.com. Dichotomy of stress response in senescent and cycling cells. Low #ribosome content in #senescence (right) reduces upstream ORF bypass (gold line) on ATF4 #RNA, despite high eIF2α-P (green). ➡️ Prevents #ISR activation and remodels the secretome (red).
#aging #cancer #GeroSky

RNAkinet identifies nascent RNA by predicting 5EU metabolically labeled #RNA from raw #nanopore electrical signal. Should allow association of RNA metabolism dynamics with isoform usage, poly(A) tails and native RNA mods. academic.oup.com/nargab/artic...

Our data also suggest an intriguing model of ISR activation that occurs in varying intensities over a continuum of ribosome content for cycling, quiescence and senescence.