Nature Reviews Genetics, Published online: 01 December 2025; doi:10.1038/s41576-025-00924-0In this Tools of the Trade article, Shubham Khetan presents PADIT-seq (protein affinity to DNA by in vitro transcription and RNA sequencing), which enables the reliable identification of low-affinity DNA binding sites for transcription factors.

Nature Reviews Genetics, Published online: 15 December 2025; doi:10.1038/s41576-025-00917-zMicrobial genomics has yielded transformative insights into the ecology and evolution of microorganisms. Nature Reviews Genetics presents a Focus issue that explores how genomic approaches reveal microbial dynamics across ecological and evolutionary contexts.

Nature Reviews Genetics, Published online: 03 December 2025; doi:10.1038/s41576-025-00912-4Ancient DNA techniques are being applied to study increasingly diverse pathogens of the past. The authors review the latest insights into pathogen–host coevolution, zoonotic events and the spread of pathogens, all while highlighting the importance of a One Health approach to this research.

Earth's c. 406 million herbarium specimens represent a largely untapped resource of genetic data that could transform our understanding of global plant populations. Advances in DNA sequencing have ma...