5/5 Together, our study introduces a powerful tool for investigating stem cell potency and offers key insights into the regulation of early embryonic development.

4/5 We also leveraged this reporter system to study regulators of cellular potency.

3/5 This metastable cell population provides a valuable tool for future investigations into both the transcriptional and post-transcriptional barriers to totipotency, as well as the molecular mechanisms underlying this state.

2/5 Unexpectedly, we found that the simultaneous expression of LTR5_Hs and MER51B marks rare, metastable primed cells. These cells are associated with DNA damage and express preimplantation-stage transcripts.

1/5 We have developed a novel dual-fluorescence reporter system that allows us to track cells during transitions between pluripotent states and stem cell differentiation at single-cell resolution.