Leonardo Almeida-Souza
@leonardoasouza.bsky.social
Professor of Cell Biology at HiLIFE, University of Helsinki 🇫🇮. 🧪 Studying endocytosis, cellular adhesions and the cytoskeleton.
http://helsinki.fi/almeida-souza-lab
Undergrad/MSc UFSCar/USP🇧🇷; PhD VIB 🇧🇪; Postdoc MRC LMB 🇬🇧
Be kind to each other
http://helsinki.fi/almeida-souza-lab
Undergrad/MSc UFSCar/USP🇧🇷; PhD VIB 🇧🇪; Postdoc MRC LMB 🇬🇧
Be kind to each other
🧵5/6 — The Big Picture
EndoNB works on endogenous proteins. No rare antibodies. No overexpression.
It’s scalable and ready to explore the 1000+ surface proteins we’ve barely studied.
We’re done understanding vesicular trafficking by looking at a handful of model surface proteins.
EndoNB works on endogenous proteins. No rare antibodies. No overexpression.
It’s scalable and ready to explore the 1000+ surface proteins we’ve barely studied.
We’re done understanding vesicular trafficking by looking at a handful of model surface proteins.
June 9, 2025 at 3:05 PM
🧵5/6 — The Big Picture
EndoNB works on endogenous proteins. No rare antibodies. No overexpression.
It’s scalable and ready to explore the 1000+ surface proteins we’ve barely studied.
We’re done understanding vesicular trafficking by looking at a handful of model surface proteins.
EndoNB works on endogenous proteins. No rare antibodies. No overexpression.
It’s scalable and ready to explore the 1000+ surface proteins we’ve barely studied.
We’re done understanding vesicular trafficking by looking at a handful of model surface proteins.
🧵3/6 — The Solution
We CRISPR-tag the protein of interest with a tiny Alfa-tag.
Then use a nanobody-SNAPtag probe with a self-destruct button (okay, a 3C protease site)
Mix them, let internalize, chill at 4°C and use 3C to cut surface signal.
What’s left? Only internalized cargo.
We CRISPR-tag the protein of interest with a tiny Alfa-tag.
Then use a nanobody-SNAPtag probe with a self-destruct button (okay, a 3C protease site)
Mix them, let internalize, chill at 4°C and use 3C to cut surface signal.
What’s left? Only internalized cargo.
June 9, 2025 at 2:59 PM
🧵3/6 — The Solution
We CRISPR-tag the protein of interest with a tiny Alfa-tag.
Then use a nanobody-SNAPtag probe with a self-destruct button (okay, a 3C protease site)
Mix them, let internalize, chill at 4°C and use 3C to cut surface signal.
What’s left? Only internalized cargo.
We CRISPR-tag the protein of interest with a tiny Alfa-tag.
Then use a nanobody-SNAPtag probe with a self-destruct button (okay, a 3C protease site)
Mix them, let internalize, chill at 4°C and use 3C to cut surface signal.
What’s left? Only internalized cargo.