@lchei.bsky.social
POTATOMap|Lysosomes|Neuronal Organelle|UU PhD Candidate|Farías lab|Utrecht University|Cell Biology|MPhil. Life Science HKUST
Pinned
lchei.bsky.social
@lchei.bsky.social
· Feb 27
Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons - Nature Communications
It is unclear how new lysosomal proteins are properly delivered in polarized neurons. Here, the authors developed a method combining the synchronization of protein release from the endoplasmic reticul...
www.nature.com
www.nature.com/articles/s41...
POTATOMap is officially out today! (Even tho it says 2024Dec30… yeah long story😂)
Thank you to all the authors and everyone involved! I hope we have provided something useful and maybe leave a little (just a little) impact in your research.
POTATOMap is officially out today! (Even tho it says 2024Dec30… yeah long story😂)
Thank you to all the authors and everyone involved! I hope we have provided something useful and maybe leave a little (just a little) impact in your research.
Reposted
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
September 22, 2025 at 6:27 PM
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Reposted
The axonal ER couples translation and secretion machineries for local delivery of axonal transmembrane proteins to promote axonal development www.biorxiv.org/content/10.1...
The axonal ER couples translation and secretion machineries for local delivery of axonal transmembrane proteins to promote axonal development
Proper neuronal development and function rely on the polarized sorting of transmembrane proteins (TMPs) to their distinct somatodendritic or axonal domains. Most TMPs follow a conventional secretory p...
www.biorxiv.org
September 10, 2025 at 1:37 PM
The axonal ER couples translation and secretion machineries for local delivery of axonal transmembrane proteins to promote axonal development www.biorxiv.org/content/10.1...
Reposted
Wow, this paper provides crucial understanding of local translation and processing of transmembrane proteins in axons, one of the big mysteries that have haunted the field for a long time!!!!!!!! Fantastic!!!!!!!! Nguyen et al., 2025, bioRxiv 2025.09.09.674816ff. -- doi.org/10.1101/2025...
September 14, 2025 at 6:12 PM
Wow, this paper provides crucial understanding of local translation and processing of transmembrane proteins in axons, one of the big mysteries that have haunted the field for a long time!!!!!!!! Fantastic!!!!!!!! Nguyen et al., 2025, bioRxiv 2025.09.09.674816ff. -- doi.org/10.1101/2025...
Reposted
Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.
September 10, 2025 at 7:18 AM
Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.
Reposted
The involvement of the ER-phagy receptor FAM134B in membrane contact sites between ER and endolysosomes promotes ERLAD https://www.biorxiv.org/content/10.1101/2025.07.08.663682v1
July 9, 2025 at 3:30 PM
The involvement of the ER-phagy receptor FAM134B in membrane contact sites between ER and endolysosomes promotes ERLAD https://www.biorxiv.org/content/10.1101/2025.07.08.663682v1
Reposted
The first review paper from our lab is out!! @viviennebauer.bsky.social discussed the rapidly emerging knowledge on (the interplay between multiple) organelles and neuronal mRNA localization. Hope you enjoy the read! www.sciencedirect.com/science/arti...
Multi-organelle-mediated mRNA localization in neurons and links to disease
Brain function requires precise spatiotemporal regulation of the neuronal proteome. To allow adaptation of the proteome in distal outposts of neurons,…
www.sciencedirect.com
March 13, 2025 at 1:38 PM
The first review paper from our lab is out!! @viviennebauer.bsky.social discussed the rapidly emerging knowledge on (the interplay between multiple) organelles and neuronal mRNA localization. Hope you enjoy the read! www.sciencedirect.com/science/arti...
www.nature.com/articles/s41...
POTATOMap is officially out today! (Even tho it says 2024Dec30… yeah long story😂)
Thank you to all the authors and everyone involved! I hope we have provided something useful and maybe leave a little (just a little) impact in your research.
POTATOMap is officially out today! (Even tho it says 2024Dec30… yeah long story😂)
Thank you to all the authors and everyone involved! I hope we have provided something useful and maybe leave a little (just a little) impact in your research.
Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons - Nature Communications
It is unclear how new lysosomal proteins are properly delivered in polarized neurons. Here, the authors developed a method combining the synchronization of protein release from the endoplasmic reticul...
www.nature.com
February 27, 2025 at 7:33 PM
www.nature.com/articles/s41...
POTATOMap is officially out today! (Even tho it says 2024Dec30… yeah long story😂)
Thank you to all the authors and everyone involved! I hope we have provided something useful and maybe leave a little (just a little) impact in your research.
POTATOMap is officially out today! (Even tho it says 2024Dec30… yeah long story😂)
Thank you to all the authors and everyone involved! I hope we have provided something useful and maybe leave a little (just a little) impact in your research.