Lori Passmore
@lapassmore.bsky.social
Molecular biologist. Group leader at MRC LMB Cambridge UK. Fellow at Clare Hall college. #CryoEM #RNAbiology #DNArepair
Views/opinions are my own.
https://www2.mrc-lmb.cam.ac.uk/group-leaders/n-to-s/lori-passmore/
Views/opinions are my own.
https://www2.mrc-lmb.cam.ac.uk/group-leaders/n-to-s/lori-passmore/
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March 2, 2025 at 9:09 PM
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Come join the amazing members of my team 🤩🤩!
November 1, 2024 at 2:39 AM
Come join the amazing members of my team 🤩🤩!
We conclude that phosphorylation can result in a rheostat-like response on the multivalent interaction allowing ‘tuning’ of deadenylation activity.
This is a common principle across multiple RNA adaptors (e.g. Pumilio proteins, TTP/ZFP36) with yeast and human deadenylation complexes.
This is a common principle across multiple RNA adaptors (e.g. Pumilio proteins, TTP/ZFP36) with yeast and human deadenylation complexes.
October 21, 2024 at 1:19 AM
We conclude that phosphorylation can result in a rheostat-like response on the multivalent interaction allowing ‘tuning’ of deadenylation activity.
This is a common principle across multiple RNA adaptors (e.g. Pumilio proteins, TTP/ZFP36) with yeast and human deadenylation complexes.
This is a common principle across multiple RNA adaptors (e.g. Pumilio proteins, TTP/ZFP36) with yeast and human deadenylation complexes.
Excitingly, this multivalency can be regulated by multi-site phosphorylation of the IDR. This modulates IDR charge and dynamics to ‘tune’ deadenylation rate.
October 21, 2024 at 1:17 AM
Excitingly, this multivalency can be regulated by multi-site phosphorylation of the IDR. This modulates IDR charge and dynamics to ‘tune’ deadenylation rate.
Targeted mutations in individual residues across the IDR (or in dispersed surface pockets of Ccr4-Not) results in a *graded* decrease in deadenylation activity, pointing towards a multivalent interaction.
October 21, 2024 at 1:17 AM
Targeted mutations in individual residues across the IDR (or in dispersed surface pockets of Ccr4-Not) results in a *graded* decrease in deadenylation activity, pointing towards a multivalent interaction.
In this work, James used NMR with @connyyu.bsky.social , crosslinking mass spec with the Rappsilber lab and biochemical reconstitution to discover that dispersed regions within the IDRs interact with multiple conserved binding sites on Ccr4-Not.
October 21, 2024 at 1:16 AM
In this work, James used NMR with @connyyu.bsky.social , crosslinking mass spec with the Rappsilber lab and biochemical reconstitution to discover that dispersed regions within the IDRs interact with multiple conserved binding sites on Ccr4-Not.
Many different ‘RNA adaptor’ proteins (RNA-binding proteins) tether specific transcripts directly to Ccr4-Not.
Intrinsically-disordered regions (IDRs) in RNA adaptors interact with Ccr4-Not. This promotes deadenylation of bound transcripts.
Intrinsically-disordered regions (IDRs) in RNA adaptors interact with Ccr4-Not. This promotes deadenylation of bound transcripts.
October 21, 2024 at 1:14 AM
Many different ‘RNA adaptor’ proteins (RNA-binding proteins) tether specific transcripts directly to Ccr4-Not.
Intrinsically-disordered regions (IDRs) in RNA adaptors interact with Ccr4-Not. This promotes deadenylation of bound transcripts.
Intrinsically-disordered regions (IDRs) in RNA adaptors interact with Ccr4-Not. This promotes deadenylation of bound transcripts.
Bluesky now has over 10 million users, and I was #1,007,137!
September 19, 2024 at 1:47 AM
Bluesky now has over 10 million users, and I was #1,007,137!