Thanks to all our collaborators and colleagues who helped make this possible, including @drcaragreen.bsky.social @mikemacarthur.bsky.social Cholsoon Jang @judisimcox.bsky.social Kristen Malecki and many more!

Diet analysis by Michael MacArthur shows that humans who score highly on the Healthy Eating Index (HEI) score have a lower relative isoleucine intake.

Importantly, by looking across different genetic backgrounds Michaela was able to identify a core set of molecular pathways engaged by IleR, providing new insights into the molecular mechanisms by which it functions.

Michaela showed that isoleucine restriction (IleR) prevents metabolic syndrome in mice fed a Western diet, with beneficial effects on metabolic health across strains and in both sexes. Conversely, excess dietary Ile has strain-specific effects on fat mass and insulin resistance.

Thanks to all our colleagues and collaborators including @drcaragreen.bsky.social, Michelle Sonsalla, Chung-Yang Yeh, @baileyknopf.bsky.social, Teresa Liu, Shoshana Yakar @sadagurski.bsky.social and many more who made this work possible!

Here is the link to the paper, demonstrating that valine restriction has robust effects on healthspan in both sexes and a sex-specific benefit for lifespan in males. www.biorxiv.org/content/10.1...

Our results suggest that not only how much we eat - but also when - may influence the development and progression of Alzheimer’s disease.

Reji asked if the benefits of CR for Alzheimer's disease also depend on fasting - and found that YES, a prolonged fast between meals is necessary for the benefits of CR, including reduced Alzheimer’s pathology, improved neuroprotective signaling, and improved cognition.

Here we build on previous work by @heidipak.bsky.social who showed that fasting between meals was required for the benefits of a calorie restricted (CR) diet on metabolic health, frailty, and lifespan. www.nature.com/articles/s42...

We thank our many colleagues and collaborators including Isaac Grunow, Reji Babygirija, Michelle Sonsalla, @baileyknopf.bsky.social, @wiscosurgicalmetab.bsky.social and Dr. Adam Konopka who helped make this work possible!

These results highlight the direct influence of dietary components on a hallmark of aging and underscore the potential for targeted dietary interventions to modulate senescence.

We further show that hepatic protection from cell senescence is cell-autonomous, with reduced BCAA levels mitigating antimycin-A-induced senescence in cultured hepatocytes.

We find that BCAA restriction not only protects male mice from the metabolic consequences of normal- and high-protein diets, we demonstrate that BCAA restriction reduces hepatic cellular senescence while enhancing senescence in white adipose tissue.

Thanks as always to our collaborators for helping to make this work possible, including Michelle Sonsalla, @drcaragreen.bsky.social Mariah Calubag, Dr. Chung Yang Yeh, Bailey Knopf, Luigi Puglielli and many more

Here, we find that dietary restriction of either leucine, isoleucine, or valine can slow the progression and development of AD pathology and preserve cognition in this mouse model of the disease. Critically, the impacts of each amino acid on AD pathology and cognition are distinct and sex-specific

That’s… not right. The concentration is also way above the (miserable low) solubility limit of rapamycin in water.

Keith came to @americanaging.bsky.social meeting several times, most recently at my invitation, and was always a delight to interact with such a brilliant and kind researcher.