Very happy to hear that! It should be quite stable for "standard" proteomics methods. Can be a bit trickier for stepping or multi-segment methods, but even these should work as far as I have tested

I sure hope that there wont be too many, haha. But I you encounter some, please report them! Happy testing :)

Yes, that should be unnecessary now!

What things did you adapt via notepad++? The polygon for dda measurements?

Thank you! Yes, also TME only works for same instrument type. timsCompare works instrument independent! Can also be a great tool to recover a dia-PASEF scheme from a PRIDE repository for example.

timsCompare is free, open-source, and available now on GitHub! All feedback, bug reports, and feature ideas are welcome.

github.com/kronigert/ti...

#MassSpec #TeamMassSpec #Proteomics #timsTOF #diaPASEF #diagonalPASEF

(Disclaimer: This is an independent project, not an official Bruker tool.)

Need to document your method for a publication or lab notebook? timsCompare generates professional, multi-page PDF reports with high-quality plots and neatly organized parameter tables. CSV export is also supported for easy data handling.

Visualize acquisition schemes for PASEF, dia-PASEF, and diagonal-PASEF methods. You can easily export these window schemes directly from your raw data into a simple text file for documentation. The exported text file can also be read directly by timsControl.

Simply drag & drop your .d or .m folders. timsCompare instantly gives you a side-by-side parameter comparison. Any differences between methods are automatically highlighted, so you can spot changes at a glance.

Will send you a document via DM! :)

Hi Dorothy, do you mean the valve plumbing or the method setup? I guess you plan to make a calibration segment at the start of each run for external calibration?

Do you want to do m/z or IM calibration?

There is a disclaimer when you scan the QR-Code. They are built from biodegradable, food-contact-safe-plastic. Please hand-wash only!

Both options are usable. For SN only directDIA is available, while TIMS DIANN uses a library-based approach

I think it's very hard to make a fair comparison between instruments. There are just too many variables

Happy to hear that everything was working out in the end! Crossing fingers for your measurements!