Kevin Keomanee-Dizon
@kevinkeomanee.bsky.social
Physicist, building new ways to see 🔬
kdizon.github.io
kdizon.github.io
New preprint 📰
We present 2P-OPM: a microscope that combines high-NA single-objective detection with two-photon plane illumination to improve spatial resolution at depth
arxiv.org/abs/2511.09462
We present 2P-OPM: a microscope that combines high-NA single-objective detection with two-photon plane illumination to improve spatial resolution at depth
arxiv.org/abs/2511.09462
November 19, 2025 at 5:10 PM
This project grew out of my independent fellowship at Princeton Physics and @cpbf.bsky.social, supported by @princeton.edu's Dean for Research Innovation Fund. Grateful to many who made this possible, especially Yaakov Clenman, Alejandra Duran, @toettch.bsky.social, and @harrymcnamara.bsky.social
November 17, 2025 at 4:22 PM
This project grew out of my independent fellowship at Princeton Physics and @cpbf.bsky.social, supported by @princeton.edu's Dean for Research Innovation Fund. Grateful to many who made this possible, especially Yaakov Clenman, Alejandra Duran, @toettch.bsky.social, and @harrymcnamara.bsky.social
The gains in resolution, contrast, and optical sectioning enable imaging in low-SNR conditions and single-molecule tracking in living fruit fly embryos, revealing how individual transcripts diffuse in their intracellular environment
November 17, 2025 at 4:22 PM
The gains in resolution, contrast, and optical sectioning enable imaging in low-SNR conditions and single-molecule tracking in living fruit fly embryos, revealing how individual transcripts diffuse in their intracellular environment
In complex multicellular systems, like organoids and gastruloids, resolution is often limited by scattering-induced out-of-focus background, not diffraction.
2P-OPM suppresses this background, maintains subcellular resolution at depth, and works with standard sample mounting
2P-OPM suppresses this background, maintains subcellular resolution at depth, and works with standard sample mounting
November 17, 2025 at 4:22 PM
In complex multicellular systems, like organoids and gastruloids, resolution is often limited by scattering-induced out-of-focus background, not diffraction.
2P-OPM suppresses this background, maintains subcellular resolution at depth, and works with standard sample mounting
2P-OPM suppresses this background, maintains subcellular resolution at depth, and works with standard sample mounting